ArticleJournal of bacteriology2011
A C-terminal region of Yersinia pestis YscD binds the outer membrane secretin YscC.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Probing Protein Topology and Conformation by Limited Proteolysis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Dynamic relocalization of cytosolic type III secretion system components prevents premature protein secretion at low external pH.Nature communications · 2021Article
- Bacterial secretins: Mechanisms of assembly and membrane targeting.Protein science : a publication of the Protein Society · 2020Review
- Cryo-EM structure of the Shigella type III needle complex.PLoS pathogens · 2020Article
- The Third Transmembrane Domain of EscR Is Critical for Function of the EnteropathogenicmSphere · 2018Article
- The extended structure of the periplasmic region of CdsD, a structural protein of the type III secretion system of Chlamydia trachomatis.Protein science : a publication of the Protein Society · 2016Article
- The Type III Secretion Translocation Pore Senses Host Cell Contact.PLoS pathogens · 2016Article
- Type Three Secretion System in Attaching and Effacing Pathogens.Frontiers in cellular and infection microbiology · 2016Review
- Building a secreting nanomachine: a structural overview of the T3SS.Current opinion in structural biology · 2014Review
- The Yersinia pestis type III secretion system: expression, assembly and role in the evasion of host defenses.Immunologic research · 2013Review
- Article
- Regulation of the Yersinia type III secretion system: traffic control.Frontiers in cellular and infection microbiology · 2013Review
- Structure and interactions of the cytoplasmic domain of the Yersinia type III secretion protein YscD.Journal of bacteriology · 2012Article
- The non-flagellar type III secretion system evolved from the bacterial flagellum and diversified into host-cell adapted systems.PLoS genetics · 2012Article
- Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.Microbiology and molecular biology reviews : MMBR · 2012Review
- Structure of the cytoplasmic domain of Yersinia pestis YscD, an essential component of the type III secretion system.Acta crystallographica. Section D, Biological crystallography · 2012Article
- Structural and functional studies on the N-terminal domain of the Shigella type III secretion protein MxiG.The Journal of biological chemistry · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
YscD is an essential component of the plasmid pCD1-encoded type III secretion system (T3SS) of Yersinia pestis. YscD has a single transmembrane (TM) domain that connects a small N-terminal cytoplasmic region (residues 1 to 121) to a larger periplasmic region (residues 143 to 419). Deletion analyses established that both the N-terminal cytoplasmic region and the C-terminal periplasmic region are required for YscD function. Smaller targeted deletions demonstrated that a predicted cytoplasmic forkhead-associated (FHA) domain is also required to assemble a functional T3SS; in contrast, a predicted periplasmic phospholipid binding (BON) domain and a putative periplasmic "ring-building motif" domain of YscD could be deleted with no significant effect on the T3S process. Although deletion of the putative "ring-building motif" domain did not disrupt T3S activity per se, the calcium-dependent regulation of the T3S apparatus was affected. The extreme C-terminal region of YscD (residues 354 to 419) was essential for secretion activity and had a strong dominant-negative effect on the T3S process when exported to the periplasm of the wild-type parent strain. Coimmunoprecipitation studies demonstrated that this region of YscD mediates the interaction of YscD with the outer membrane YscC secretin complex. Finally, replacement of the YscD TM domain with a TM domain of dissimilar sequence had no effect on the T3S process, indicating that the TM domain has no sequence-specific function in the assembly or function of the T3SS.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.