ArticleEukaryotic cell2011
Wsp1, a GBD/CRIB domain-containing WASP homolog, is required for growth, morphogenesis, and virulence of Cryptococcus neoformans.
Article in Eukaryotic cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Genetic Transformation inJournal of fungi (Basel, Switzerland) · 2021Review
- Transcriptomic Analysis of Extracellular RNA Governed by the Endocytic Adaptor Protein Cin1 ofFrontiers in cellular and infection microbiology · 2020Article
- The TOR Pathway Plays Pleiotropic Roles in Growth and Stress Responses of the Fungal PathogenGenetics · 2019Article
- Role of clathrin-mediated endocytosis in the use of heme and hemoglobin by the fungal pathogen Cryptococcus neoformans.Cellular microbiology · 2019Article
- Role of Downregulation and Phosphorylation of Cofilin in Polarized Growth, MpkA Activation and Stress Response ofFrontiers in microbiology · 2018Article
- Comparative Ecology of CapsularFrontiers in microbiology · 2017Article
- Two Rac paralogs regulate polarized growth in the human fungal pathogen Cryptococcus neoformans.Fungal genetics and biology : FG & B · 2013Article
- Aimless mutants of Cryptococcus neoformans: failure to disseminate.Fungal biology reviews · 2012Article
- The Cryptococcus neoformans capsule: a sword and a shield.Clinical microbiology reviews · 2012Review
- Wsp1 is downstream of Cin1 and regulates vesicle transport and actin cytoskeleton as an effector of Cdc42 and Rac1 in Cryptococcus neoformans.Eukaryotic cell · 2012Article
- Ras-Mediated Signal Transduction and Virulence in Human Pathogenic Fungi.Fungal genomics & biology · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Human endocytic protein ITSN1 regulates actin reorganization by activating Rho family GTPases, such as Cdc42. The process is enhanced by ITSN binding of WASP, an effector of Cdc42 and a potent activator of actin polymerization. In the human pathogen Cryptococcus neoformans, endocytic protein Cin1 also interacts with Cdc42 and Wsp1, an uncharacterized WASP homolog, but the significance of these interactions remains unknown. Wsp1 contains several conserved domains, including a WASP homology 1 domain (WH1), a GTPase binding/Cdc42 and Rac interactive binding domain (GBD/CRIB), and a C-terminal domain composed of verprolin-like, central, and acidic motifs (VCA). Thus, Wsp1 exhibits domain compositions more similar to human WASP proteins than Saccharomyces cerevisiae Las17/Bee1, a WASP homolog lacking the GDB/CRIB domain. Wsp1 is not an essential protein; however, the wsp1 mutant exhibited defects in growth, cytokinesis, chitin distribution, and endocytosis and exocytosis. The wsp1 mutant was also unable to undergo genetic cross, produce the polysaccharide capsule, or secrete the enzyme urease. An in vitro phagocytosis assay showed a higher phagocytic index for the wsp1 mutant, whose ability to cause lethal infection in a murine model of cryptococcosis was also attenuated. Our studies reveal divergent evolution of WASP proteins in the fungal phylum and suggest that the conserved function of WASP proteins in the actin cytoskeleton may also impact fungal virulence.
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