ArticleApplied and environmental microbiology2011
Detecting potentially virulent Vibrio vulnificus strains in raw oysters by quantitative loop-mediated isothermal amplification.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- A Fluorogenic Biosensor for Direct Detection of Vibrio vulnificus, a Climate Change Biomarker.MicrobiologyOpen · 2026Article
- Article
- Occurrence and Molecular Characterization of Potentially PathogenicMicroorganisms · 2022Article
- Article
- Simultaneous isolation and enumeration of virulent Vibrio cholerae and Vibrio vulnificus using an advanced MPN-PCR method.Archives of microbiology · 2021Article
- Development and evaluation of loop-mediated isothermal amplification for detection of Yersinia pestis in plague biological samples.PloS one · 2020Article
- GLAPD: Whole Genome Based LAMP Primer Design for a Set of Target Genomes.Frontiers in microbiology · 2019Article
- Preliminary study on the antimicrobial susceptibility pattern related to the genotype ofItalian journal of food safety · 2017Article
- Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7.Journal of visualized experiments : JoVE · 2017Article
- Multiparametric Magneto-fluorescent Nanosensors for the Ultrasensitive Detection of Escherichia coli O157:H7.ACS infectious diseases · 2016Article
- First Multi-Year Retrospective Study onItalian journal of food safety · 2016Article
- Occurrence of VibrioItalian journal of food safety · 2016Article
- Loop-mediated isothermal amplification for Rickettsia typhi (the causal agent of murine typhus): problems with diagnosis at the limit of detection.Journal of clinical microbiology · 2014Article
- Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations.Frontiers in microbiology · 2014Review
- Rapid and specific detection of escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157 in ground beef, beef trim, and produce by loop-mediated isothermal amplification.Applied and environmental microbiology · 2012Article
- Loop-mediated isothermal amplification assays for detecting shiga toxin-producing Escherichia coli in ground beef and human stools.Journal of clinical microbiology · 2012Article
- Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification.Applied and environmental microbiology · 2011Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Vibrio vulnificus is a leading cause of seafood-related deaths in the United States. Sequence variations in the virulence-correlated gene (vcg) have been used to distinguish between clinical and environmental V. vulnificus strains, with a strong association between clinical ones and the C sequence variant (vcgC). In this study, vcgC was selected as the target to design a loop-mediated isothermal amplification (LAMP) assay for the rapid, sensitive, specific, and quantitative detection of potentially virulent V. vulnificus strains in raw oysters. No false-positive or false-negative results were generated among the 125 bacterial strains used to evaluate assay specificity. The detection limit was 5.4 CFU per reaction for a virulent V. vulnificus strain (ATCC 33815) in pure culture, 100-fold more sensitive than that of PCR. In spiked raw oysters, the assay was capable of detecting 2.5 × 10(3) CFU/g of V. vulnificus ATCC 33815, while showing negative results for a nonvirulent V. vulnificus strain (515-4c2) spiked at 10(7) CFU/g. After 6 h of enrichment, the LAMP assay could detect 1 CFU/g of the virulent V. vulnificus strain ATCC 33815. Standard curves generated in pure culture and spiked oysters suggested a good linear relationship between cell numbers of the virulent V. vulnificus strain and turbidity signals. In conclusion, the LAMP assay developed in this study could quantitatively detect potentially virulent V. vulnificus in raw oysters with high speed, specificity, and sensitivity, which may facilitate better control of V. vulnificus risks associated with raw oyster consumption.
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