ArticleThe Journal of biological chemistry2011
Specific lipids modulate the transporter associated with antigen processing (TAP).
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Defining the heterogeneous composition of Arabidopsis thylakoid membrane.The Plant journal : for cell and molecular biology · 2025Article
- Chemical tools for membrane protein structural biology.Current opinion in structural biology · 2019Review
- Peptide translocation by the lysosomal ABC transporter TAPL is regulated by coupling efficiency and activation energy.Scientific reports · 2019Article
- Emerging Diversity in Lipid-Protein Interactions.Chemical reviews · 2019Review
- Easy, Fast, and Reproducible Quantification of Cholesterol and Other Lipids in Human Plasma by Combined High Resolution MSX and FTMS Analysis.Journal of the American Society for Mass Spectrometry · 2018Article
- Moving the Cellular Peptidome by Transporters.Frontiers in cell and developmental biology · 2018Review
- Article
- A subset of annular lipids is linked to the flippase activity of an ABC transporter.Nature chemistry · 2015Article
- A mutation within the extended X loop abolished substrate-induced ATPase activity of the human liver ATP-binding cassette (ABC) transporter MDR3.The Journal of biological chemistry · 2015Article
- A negative feedback modulator of antigen processing evolved from a frameshift in the cowpox virus genome.PLoS pathogens · 2014Article
- An annular lipid belt is essential for allosteric coupling and viral inhibition of the antigen translocation complex TAP (transporter associated with antigen processing).The Journal of biological chemistry · 2014Article
- Structures of ABCB10, a human ATP-binding cassette transporter in apo- and nucleotide-bound states.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Multicolour fluorescence-detection size-exclusion chromatography for structural genomics of membrane multiprotein complexes.PloS one · 2013Article
- A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris.PloS one · 2011Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transporter associated with antigen processing (TAP) plays a key role in adaptive immunity by translocating proteasomal degradation products from the cytosol into the endoplasmic reticulum lumen for subsequent loading onto major histocompatibility (MHC) class I molecules. For functional and structural analysis of this ATP-binding cassette complex, we established the overexpression of TAP in the methylotrophic yeast Pichia pastoris. Screening of optimal solubilization and purification conditions allowed the isolation of the heterodimeric transport complex, yielding 30 mg of TAP/liter of culture. Detailed analysis of TAP function in the membrane, solubilized, purified, and reconstituted states revealed a direct influence of the native lipid environment on activity. TAP-associated phospholipids, essential for function, were profiled by liquid chromatography Fourier transform mass spectrometry. The antigen translocation activity is stimulated by phosphatidylinositol and -ethanolamine, whereas cholesterol has a negative effect on TAP activity.
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