ArticleThe Journal of biological chemistry2011
G protein-coupled receptor kinase-2 is a novel regulator of collagen synthesis in adult human cardiac fibroblasts.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Mechanotransduction in Wound Healing and Fibrosis.Journal of clinical medicine · 2020Review
- Functional cardiac fibroblasts derived from human pluripotent stem cells via second heart field progenitors.Nature communications · 2019Article
- Cardiac Fibrosis: The Fibroblast Awakens.Circulation research · 2016Review
- SIRT3 Blocks Aging-Associated Tissue Fibrosis in Mice by Deacetylating and Activating Glycogen Synthase Kinase 3β.Molecular and cellular biology · 2015Article
- Regulation of mitochondrial oxidative stress by β-arrestins in cultured human cardiac fibroblasts.Disease models & mechanisms · 2015Article
- In Vitro Evaluation of Achillea Millefolium on the Production and Stimulation of Human Skin Fibroblast Cells (HFS-PI-16).Medical archives (Sarajevo, Bosnia and Herzegovina) · 2015Article
- An updated review of mechanotransduction in skin disorders: transcriptional regulators, ion channels, and microRNAs.Cellular and molecular life sciences : CMLS · 2015Review
- Neuronal NF1/RAS regulation of cyclic AMP requires atypical PKC activation.Human molecular genetics · 2014Article
- GRK2 in the heart: a GPCR kinase and beyond.Antioxidants & redox signaling · 2014Review
- β-Arrestins regulate human cardiac fibroblast transformation and collagen synthesis in adverse ventricular remodeling.Journal of molecular and cellular cardiology · 2014Article
- Dynamic mass redistribution analysis of endogenous β-adrenergic receptor signaling in neonatal rat cardiac fibroblasts.Pharmacology research & perspectives · 2014Article
- Adenosine 2A receptor promotes collagen production by human fibroblasts via pathways involving cyclic AMP and AKT but independent of Smad2/3.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Developmental and tumoral vascularization is regulated by G protein-coupled receptor kinase 2.The Journal of clinical investigation · 2013Article
- GRK 2 level in peripheral blood lymphocytes of elderly patients with acute myocardial infarction.Journal of geriatric cardiology : JGC · 2013Article
- Revealing the mechanism of in vitro wound healing properties of Citrus tamurana extract.BioMed research international · 2013Article
- GRK2-mediated inhibition of adrenergic and dopaminergic signaling in right ventricular hypertrophy: therapeutic implications in pulmonary hypertension.Circulation · 2012Article
- cAMP and Epac in the regulation of tissue fibrosis.British journal of pharmacology · 2012Review
- Wound healing properties of ethyl acetate fraction of Moringa oleifera in normal human dermal fibroblasts.Journal of intercultural ethnopharmacologyArticle
Corrections and comments
- Retraction · 2015-10-23Falsification/Fabrication of Data · Falsification/Fabrication of Image · Investigation by Company/Institution · Investigation by ORI · Misconduct - Official Investigation(s) and/or Finding(s) · Misconduct by Author · · Ricky Malhotra had NIH finding of misconduct - https://ori.hhs.gov/content/case-summary-malhotra-ricky; Karen D'Souza has NIH finding of misconduct
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- Retracted
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Cardiac fibroblasts (CF) make up 60-70% of the total cell number in the heart and play a critical role in regulating normal myocardial function and in adverse remodeling following myocardial infarction and the transition to heart failure. Recent studies have shown that increased intracellular cAMP can inhibit CF transformation and collagen synthesis in adult rat CF; however, mechanisms by which cAMP production is regulated in CF have not been elucidated. We investigated the potential role of G protein-coupled receptor kinase-2 (GRK2) in modulating collagen synthesis by adult human CF isolated from normal and failing left ventricles. Baseline collagen synthesis was elevated in failing CF and was not inhibited by β-agonist stimulation in contrast to normal controls. β-adrenergic receptor (β-AR) signaling was markedly uncoupled in the failing CF, and expression and activity of GRK2 were increased 3-fold. Overexpression of GRK2 in normal CF recapitulated a heart failure phenotype with minimal inhibition of collagen synthesis following β-agonist stimulation. In contrast, knockdown of GRK2 expression in normal CF enhanced cAMP production and led to greater β-agonist-mediated inhibition of basal and TGFβ-stimulated collagen synthesis versus control. Inhibition of GRK2 activity in failing CF by expression of the GRK2 inhibitor, GRK2ct, or siRNA-mediated knockdown restored β-agonist-stimulated inhibition of collagen synthesis and decreased collagen synthesis in response to TGFβ stimulation. GRK2 appears to play a significant role in regulating collagen synthesis in adult human CF, and increased activity of this kinase may be an important mechanism of maladaptive ventricular remodeling as mediated by cardiac fibroblasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.