ArticleThe Journal of biological chemistry2011
The essential Ubc4/Ubc5 function in yeast is HECT E3-dependent, and RING E3-dependent pathways require only monoubiquitin transfer by Ubc4.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Proteome Profiling ofMicroorganisms · 2024Article
- The role of ubiquitin-conjugating enzyme in the process of spermatogenesis.Reproductive biology and endocrinology : RB&E · 2024Review
- Ubiquitin-Conjugating Enzymes Ubc1 and Ubc4 Mediate the Turnover of Hap4, a Master Regulator of Mitochondrial Biogenesis inMicroorganisms · 2022Article
- The UBA domain of conjugating enzyme Ubc1/Ube2K facilitates assembly of K48/K63-branched ubiquitin chains.The EMBO journal · 2021Article
- Protein quality control degron-containing substrates are differentially targeted in the cytoplasm and nucleus by ubiquitin ligases.Genetics · 2021Article
- Growth Inhibition by Amino Acids inMicroorganisms · 2020Article
- Ubiquitin-proteasome-mediated cyclin C degradation promotes cell survival following nitrogen starvation.Molecular biology of the cell · 2020Article
- Article
- Determinants of the cytosolic turnover of mitochondrial intermembrane space proteins.BMC biology · 2018Article
- Convergent evolution in the assembly of polyubiquitin degradation signals by the Shigella flexneri IpaH9.8 ligase.The Journal of biological chemistry · 2014Article
- Specific α-arrestins negatively regulate Saccharomyces cerevisiae pheromone response by down-modulating the G-protein-coupled receptor Ste2.Molecular and cellular biology · 2014Article
- The ubiquitin-conjugating enzyme, UbcM2, is restricted to monoubiquitylation by a two-fold mechanism that involves backside residues of E2 and Lys48 of ubiquitin.Biochemistry · 2014Article
- E3 ubiquitin ligase Pub1 is implicated in endocytosis of a GPI-anchored protein Ecm33 in fission yeast.PloS one · 2014Article
- Previously unknown role for the ubiquitin ligase Ubr1 in endoplasmic reticulum-associated protein degradation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- STUbLs in chromatin and genome stability.Biopolymers · 2013Review
- Structural determinants of ubiquitin conjugation in Entamoeba histolytica.The Journal of biological chemistry · 2013Article
- The ubiquitin-proteasome system of Saccharomyces cerevisiae.Genetics · 2012Review
- The prolific ATL family of RING-H2 ubiquitin ligases.Plant signaling & behavior · 2012Review
- Mechanisms for countering oxidative stress and damage in retinal pigment epithelium.International review of cell and molecular biology · 2012Review
- Cellular strategies for making monoubiquitin signals.Critical reviews in biochemistry and molecular biologyReview
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4 authors at 1 institution in 1 country.
Funding
Abstract
The ubiquitin (Ub)-conjugating enzymes Ubc4 and Ubc5 are involved in a variety of ubiquitination pathways in yeast, including Rsp5- and anaphase-promoting complex (APC)-mediated pathways. We have found the double deletion of UBC4 and UBC5 genes in yeast to be lethal. To investigate the essential pathway disrupted by the ubc4/ubc5 deletion, several point mutations were inserted in Ubc4. The Ubc4 active site mutation C86A and the E3-binding mutations A97D and F63A were both unable to rescue the lethal phenotype, indicating that an active E3/E2∼Ub complex is required for the essential function of Ubc4/Ubc5. A mutation that specifically eliminates RING E3-catalyzed isopeptide formation but not HECT E3 transthiolation (N78S-Ubc4) rescued the lethal phenotype. Thus, the essential redundant function performed by Ubc4 and Ubc5 in yeast is with a HECT-type E3, likely the only essential HECT in yeast, Rsp5. Our results also suggest that Ubc1 can weakly replace Ubc4 to transfer mono-Ub with APC, but Ubc4 cannot replace Ubc1 for poly-Ub chain extension on APC substrates. Finally, the backside Ub-binding mutant S23R-Ubc4 has no observable effect in yeast. Together, our results are consistent with a model in which Ubc4 and Ubc5 are 1) the primary E2s for Rsp5 in yeast and 2) act as monoubiquitinating E2s in RING E3-catalyzed pathways, in contrast to the processive human ortholog UbcH5.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.