ArticleMolecular plant pathology2011
The reliability of virus-induced gene silencing experiments using tobacco rattle virus in tomato is influenced by the size of the vector control.
Article in Molecular plant pathology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Virus-induced gene silencing as a tool for functional genomics in weeds: Challenges and future directions.Genetics and molecular biology · 2026Article
- RNA interference: a promising biotechnological approach to combat plant pathogens, mechanism and future prospects.World journal of microbiology & biotechnology · 2024Review
- Integrating cotyledon-based virus-induced gene silencing with visual marker promises a rapid, highly effective validation of gene functions inFrontiers in plant science · 2024Article
- Members of the ribosomal protein S6 (RPS6) family act as pro-viral factor for tomato spotted wilt orthotospovirus infectivity in Nicotiana benthamiana.Molecular plant pathology · 2022Article
- Genome-Wide Identification of Cotton (Plants (Basel, Switzerland) · 2022Article
- Impairment of TomatoFrontiers in plant science · 2021Article
- Meiotic crossover reduction by virus-induced gene silencing enables the efficient generation of chromosome substitution lines and reverse breeding in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2020Article
- Central role of 70-kDa heat shock protein in adaptation of plants to drought stress.Cell stress & chaperones · 2020Article
- Identification of transcription factors controlling floral morphology in wild Petunia species with contrasting pollination syndromes.The Plant journal : for cell and molecular biology · 2020Article
- Development of a Virus-Induced Gene Silencing System for Dioecious Coccinia grandis.Molecular biotechnology · 2020Article
- ThePlant physiology · 2020Article
- NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves.Plants (Basel, Switzerland) · 2019Article
- A Plant Virus-Based Vector System for Gene Function Studies in Pepper.Plant physiology · 2019Article
- Tomato PEPR1 ORTHOLOG RECEPTOR-LIKE KINASE1 Regulates Responses to Systemin, Necrotrophic Fungi, and Insect Herbivory.The Plant cell · 2018Article
- Article
- The ethylene response factor Pti5 contributes to potato aphid resistance in tomato independent of ethylene signalling.Journal of experimental botany · 2015Article
- Tobacco rattle virus-based virus-induced gene silencing in Nicotiana benthamiana.Nature protocols · 2014Article
- An Optimized Protocol to Increase Virus-Induced Gene Silencing Efficiency and Minimize Viral Symptoms in Petunia.Plant molecular biology reporter · 2014Article
- Loss of function of FATTY ACID DESATURASE7 in tomato enhances basal aphid resistance in a salicylate-dependent manner.Plant physiology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tobacco rattle virus (TRV)-based vector pYL156 is the most widely used vector for virus-induced gene silencing (VIGS) in solanaceous plants. We show here that vacuum agroinfiltration of the pYL156 empty vector causes extensive stem lesions, foliar necrosis, stunted growth and delayed flowering in tomato, whereas plants infiltrated with pYL156-based silencing constructs that carry inserts of ∼250 bp or more show few symptoms of virus infection. This difference in plant health influences plant susceptibility to insects, a trait measured in many VIGS experiments, and could potentially bias the results of other bioassays designed to assess the phenotypic consequences of VIGS. When plants were infiltrated with the pYL156:GUS construct, which carries a 396-bp fragment of the β-glucuronidase gene that has no significant homology with any endogenous gene in tomato, they showed similar growth and development as buffer-infiltrated controls, and exhibited significantly fewer viral symptoms than the empty vector. Furthermore, pYL156:GUS did not influence plant susceptibility to aphids. Reverse transcription-polymerase chain reaction (RT-PCR) results indicated that the GUS insert may cause a modest delay in virus movement within the plant, but does not limit TRV replication in infected tissue. Therefore, the pYL156:GUS construct represents an improved negative control for TRV-induced gene silencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.