ArticleAnalytical chemistry2011
Microwell device for targeting single cells to electrochemical microelectrodes for high-throughput amperometric detection of quantal exocytosis.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
- Article
- Impedance spectroscopy of the cell/nanovolcano interface enables optimization for electrophysiology.Microsystems & nanoengineering · 2023Article
- Instrumented Microphysiological Systems for Real-Time Measurement and Manipulation of Cellular Electrochemical Processes.iScience · 2019Review
- Dielectrophoresis Multipath Focusing of Microparticles through Perforated Electrodes in Microfluidic Channels.Biosensors · 2019Article
- Single-Cell Recording of Vesicle Release From Human Neuroblastoma Cells Using 1024-ch Monolithic CMOS Bioelectronics.IEEE transactions on biomedical circuits and systems · 2018Article
- Polydopamine Surface Chemistry: A Decade of Discovery.ACS applied materials & interfaces · 2018Article
- Electrochemical measurement of quantal exocytosis using microchips.Pflugers Archiv : European journal of physiology · 2018Review
- A matched-filter algorithm to detect amperometric spikes resulting from quantal secretion.Journal of neuroscience methods · 2018Article
- Surface-modified CMOS IC electrochemical sensor array targeting single chromaffin cells for highly parallel amperometry measurements.Pflugers Archiv : European journal of physiology · 2018Review
- Two approaches for addressing electrochemical electrode arrays with reduced external connections.Analytical methods : advancing methods and applications · 2015Article
- Microfluidic device with tunable post arrays and integrated electrodes for studying cellular release.The Analyst · 2014Article
- Fully integrated CMOS microsystem for electrochemical measurements on 32 × 32 working electrodes at 90 frames per second.Analytical chemistry · 2014Article
- Spatial resolution of single-cell exocytosis by microwell-based individually addressable thin film ultramicroelectrode arrays.Analytical chemistry · 2014Article
- Nanocavity crossbar arrays for parallel electrochemical sensing on a chip.Beilstein journal of nanotechnology · 2014Article
- A microfluidic platform for chemical stimulation and real time analysis of catecholamine secretion from neuroendocrine cells.Lab on a chip · 2013Article
- Selective catecholamine recognition with NeuroSensor 521: a fluorescent sensor for the visualization of norepinephrine in fixed and live cells.ACS chemical neuroscience · 2013Article
- Electroporation followed by electrochemical measurement of quantal transmitter release from single cells using a patterned microelectrode.Lab on a chip · 2013Article
- Fabrication of two-layer poly(dimethyl siloxane) devices for hydrodynamic cell trapping and exocytosis measurement with integrated indium tin oxide microelectrodes arrays.Biomedical microdevices · 2013Article
- Microchip-based electrochemical detection for monitoring cellular systems.Analytical and bioanalytical chemistry · 2013Review
- Parallel recording of neurotransmitters release from chromaffin cells using a 10×10 CMOS IC potentiostat array with on-chip working electrodes.Biosensors & bioelectronics · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Electrochemical microelectrodes are commonly used to detect spikes of amperometric current that correspond to exocytosis of oxidizable transmitter from individual vesicles, i.e., quantal exocytosis. We are developing transparent multielectrochemical electrode arrays on microchips in order to automate measurement of quantal exocytosis. Here, we report development of an improved device to target individual cells to each microelectrode in an array. Efficient targeting (~75%) is achieved using cell-sized microwell traps fabricated in SU-8 photoresist together with patterning of poly(l-lysine) in register with electrodes to promote cell adhesion. The surface between electrodes is made resistant to cell adhesion using poly(ethylene glycol) in order to facilitate movement of cells to electrode "docking sites". We demonstrate the activity of the electrodes using the test analyte ferricyanide and perform recordings of quantal exocytosis from bovine adrenal chromaffin cells on the device. Multiple cell recordings on a single device demonstrate the consistency of spike measurements, and multiple recordings from the same electrodes demonstrate that the device can be cleaned and reused without degradation of performance. The new device will enable high-throughput studies of quantal exocytosis and may also find application in rapidly screening drugs or toxins for effects on exocytosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.