ArticleClinical cancer research : an official journal of the American Association for Cancer Research2011
Intraoperative qRT-PCR for detection of lymph node metastasis in head and neck cancer.
Article in Clinical cancer research : an official journal of the American Association for Cancer Research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
- Comparison of real-time PCR and nCounter NanoString techniques to validate copy number alterations in oral cancer.Scientific reports · 2025Article
- A pilot study of quantitative real-time polymerase chain reaction metastases detection on sentinel lymph nodes of oral cancer and literature review.Revista da Associacao Medica Brasileira (1992) · 2024Review
- Ultra-rapid somatic variant detection via real-time targeted amplicon sequencing.Communications biology · 2022Article
- Oxygen Imaging for Non-Invasive Metastasis Detection.Sensors (Basel, Switzerland) · 2021Article
- Paradoxical Roles of Desmosomal Components in Head and Neck Cancer.Biomolecules · 2021Review
- Detecting cancer metastasis and accompanying protein biomarkers at single cell levels using a 3D-printed microfluidic immunoarray.Biosensors & bioelectronics · 2021Article
- Development of a four-gene prognostic model for pancreatic cancer based on transcriptome dysregulation.Aging · 2020Article
- Identification of molecular biomarkers for the diagnosis of gastric cancer and lymph-node metastasis.Gastroenterology report · 2019Article
- Invasion-Related Factors as Potential Diagnostic and Therapeutic Targets in Oral Squamous Cell Carcinoma-A Review.International journal of molecular sciences · 2018Review
- Intraoperative Sentinel Lymph Node Evaluation: Implications of Cytokeratin 19 Expression for the Adoption of OSNA in Oral Squamous Cell Carcinoma.Annals of surgical oncology · 2016Article
- The Role of Sentinel Lymph Node Biopsy in Head and Neck Cancers and Its Application Areas.Turkish archives of otorhinolaryngology · 2016Review
- Desmogleins as prognostic biomarkers in resected pancreatic ductal adenocarcinoma.British journal of cancer · 2015Article
- Accuracy of microRNAs as markers for the detection of neck lymph node metastases in patients with head and neck squamous cell carcinoma.BMC medicine · 2015Article
- CD147 and Ki-67 overexpression confers poor prognosis in squamous cell carcinoma of oral tongue: a tissue microarray study.Oral surgery, oral medicine, oral pathology and oral radiology · 2015Article
- Review
- Article
- Correlation of tumor marker expression with nodal disease burden in metastatic head and neck cancer.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2013Article
- Prognostic biological features in neck dissection specimens.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2013Review
- DSG3 as a biomarker for the ultrasensitive detection of occult lymph node metastasis in oral cancer using nanostructured immunoarrays.Oral oncology · 2013Article
- Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells.Stem cell research & therapy · 2013Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
Abstract
purposeSentinel node biopsy (SNB) has been shown to accurately stage the regional lymphatics in oral carcinoma. However, intraoperative pathology is only moderately sensitive and final pathology takes several days to complete. The purpose of this study was to develop a rapid, automated, and quantitative real-time PCR (qRT-PCR) assay that can match final pathology in an intraoperative time frame. EXPERIMENTAL
designFour hundred forty-eight grossly tumor-negative lymph nodes were evaluated for expression of 3 markers [PVA (pemphigus vulgaris antigen), PTHrP (parathyroid hormone-related protein), and TACSTD1 (tumor-associated calcium signal transducer 1)]. Conformity of metastasis detection by qRT-PCR was determined using hematoxylin and eosin and immunohistochemistry staining as the gold standard. PVA and TACSTD1 were then multiplexed with β-glucuronidase to develop a rapid, automated single-tube qRT-PCR assay using the Cepheid GeneXpert system. This assay was used to analyze 103 lymph nodes in an intraoperative time frame.
resultsFour hundred forty-two nodes produced an informative result for both qRT-PCR and pathologic examination. Concordance of qRT-PCR for individual markers with final pathology ranged from 93% to 98%. The best marker combination was TACSTD1 and PVA. A rapid, multiplex assay for TACSTD1 and PVA was developed on the Cepheid GeneXpert and demonstrated an excellent reproducibility and linearity. Analysis of 103 lymph nodes demonstrated 94.2% accuracy of this assay for identifying positive and negative nodes. The average time for each assay to yield results was 35 minutes.
conclusionsA rapid, automated qRT-PCR assay can detect lymph node metastasis in head and neck cancer with high accuracy compared to pathologic analysis and may be more accurate than intraoperative pathology. Combined, SNB and rapid qRT-PCR could more appropriately guide surgical treatment of patients with head and neck cancer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.