ArticleNucleic acids research2011
Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
- Real-Time Assessment of Intracellular Metabolites in Single Cells through RNA-Based Sensors.Biomolecules · 2023Review
- Aptamers, Riboswitches, and Ribozymes inLife (Basel, Switzerland) · 2021Review
- In Vivo Validation of a Reversible Small Molecule-Based Switch for Synthetic Self-Amplifying mRNA Regulation.Molecular therapy : the journal of the American Society of Gene Therapy · 2021Article
- Ligand-dependent tRNA processing by a rationally designed RNase P riboswitch.Nucleic acids research · 2021Article
- Tuning response curves for synthetic biology.ACS synthetic biology · 2013Article
- De novo design of a synthetic riboswitch that regulates transcription termination.Nucleic acids research · 2013Article
- Synthetic biology: advancing the design of diverse genetic systems.Annual review of chemical and biomolecular engineering · 2013Review
- Synthetic RNA switches as a tool for temporal and spatial control over gene expression.Current opinion in biotechnology · 2012Review
- Rational design of allosteric inhibitors and activators using the population-shift model: in vitro validation and application to an artificial biosensor.Journal of the American Chemical Society · 2012Article
- Synthetic RNA modules for fine-tuning gene expression levels in yeast by modulating RNase III activity.Nucleic acids research · 2011Article
- Engineering biological systems with synthetic RNA molecules.Molecular cell · 2011Review
- Article
- Ribonuclease III mechanisms of double-stranded RNA cleavage.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The programming of cellular networks to achieve new biological functions depends on the development of genetic tools that link the presence of a molecular signal to gene-regulatory activity. Recently, a set of engineered RNA controllers was described that enabled predictable tuning of gene expression in the yeast Saccharomyces cerevisiae through directed cleavage of transcripts by an RNase III enzyme, Rnt1p. Here, we describe a strategy for building a new class of RNA sensing-actuation devices based on direct integration of RNA aptamers into a region of the Rnt1p hairpin that modulates Rnt1p cleavage rates. We demonstrate that ligand binding to the integrated aptamer domain is associated with a structural change sufficient to inhibit Rnt1p processing. Three tuning strategies based on the incorporation of different functional modules into the Rnt1p switch platform were demonstrated to optimize switch dynamics and ligand responsiveness. We further demonstrated that these tuning modules can be implemented combinatorially in a predictable manner to further improve the regulatory response properties of the switch. The modularity and tunability of the Rnt1p switch platform will allow for rapid optimization and tailoring of this gene control device, thus providing a useful tool for the design of complex genetic networks in yeast.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.