ArticleBiophysical journal2011
In vivo imaging of myelin in the vertebrate central nervous system using third harmonic generation microscopy.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed.
- Atto 643 Carboxy Selectively Labels Astrocytes with Minimal Oligodendrocyte Cross-Reactivity.bioRxiv : the preprint server for biology · 2026Article
- Brain-wide mapping of oligodendrocyte organization, oligodendrogenesis, and myelin injury.Cell · 2026Article
- Label-free tracking of subcortical white matter degradationBiomedical optics express · 2026Article
- Advances in multiscale myelin imaging: from classical histology to functional insights.Frontiers in cellular neuroscience · 2026Review
- Myelinated fiber labeling and orientation mapping of the human brain with light-sheet fluorescence microscopy.NeuroImage · 2025Article
- Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue.Communications biology · 2025Article
- Birefringence microscopy enables rapid, label-free quantification of myelin debris following induced cortical injury.Neurophotonics · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- Label-Free Multiphoton Imaging Reveals Volumetric Shifts Across Development in Sensory-Related Brain Regions of a Miniature Transparent Vertebrate.The Journal of comparative neurology · 2025Article
- Dynamic imaging of myelin pathology in physiologically preserved human brain tissue using third harmonic generation microscopy.PloS one · 2025Article
- Three-dimensional fiber orientation mapping of ex vivo human brain at micrometer resolution.Npj imaging · 2025Article
- Optimized intravital three-photon imaging of intact mouse tibia links plasma cell motility to functional states.iScience · 2024Article
- Emerging Functional Connections Between Metabolism and Epigenetic Remodeling in Neural Differentiation.Molecular neurobiology · 2024Review
- Label-free, whole-brain in vivo mapping in an adult vertebrate with third harmonic generation microscopy.The Journal of comparative neurology · 2024Article
- Vibrational spectroscopy and multiphoton microscopy for label-free visualization of nervous system degeneration and regeneration.Biophysical reviews · 2024Review
- Live Mapping of the Brain Extracellular Matrix and Remodeling in Neurological Disorders.Small methods · 2024Article
- Practical considerations for birefringence microscopy of myelin structure: Microscope design and tissue processing for effective imaging.Imaging neuroscience (Cambridge, Mass.) · 2024Article
- Imaging Approaches to Investigate Pathophysiological Mechanisms of Brain Disease in Zebrafish.International journal of molecular sciences · 2023Review
- Third harmonic imaging contrast from tubular structures in the presence of index discontinuity.Scientific reports · 2023Article
- Label-free intraoperative nerve detection and visualization using ratiometric diffuse reflectance spectroscopy.Scientific reports · 2023Article
25 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
Loss of myelin in the central nervous system (CNS) leads to debilitating neurological deficits. High-resolution optical imaging of myelin in the CNS of animal models is limited by a lack of in vivo myelin labeling strategies. We demonstrated that third harmonic generation (THG) microscopy-a coherent, nonlinear, dye-free imaging modality-provides micrometer resolution imaging of myelin in the mouse CNS. In fixed tissue, we found that THG signals arose from white matter tracts and were colocalized with two-photon excited fluorescence (2PEF) from a myelin-specific dye. In vivo, we used simultaneous THG and 2PEF imaging of the mouse spinal cord to resolve myelin sheaths surrounding individual fluorescently-labeled axons, and followed myelin disruption after spinal cord injury. Finally, we suggest optical mechanisms that underlie the myelin specificity of THG. These results establish THG microscopy as an ideal tool for the study of myelin loss and recovery.
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