ArticleBiophysical journal2011
Bilayer-mediated clustering and functional interaction of MscL channels.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed.
- Cell-free expression with a quartz crystal microbalance enables rapid, dynamic, and label-free characterization of membrane-interacting proteins.Communications biology · 2024Article
- The principles and promising future of sonogenetics for precision medicine.Theranostics · 2024Review
- Length matters: Functional flip of the short TatA transmembrane helix.Biophysical journal · 2023Article
- Article
- Modulation of deep neural circuits with sonogenetics.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- "Force-From-Lipids" Dependence of the MscCG Mechanosensitive Channel Gating on Anionic Membranes.Microorganisms · 2023Article
- Solid and Liquid Surface-Supported Bacterial Membrane Mimetics as a Platform for the Functional and Structural Studies of Antimicrobials.Membranes · 2022Review
- Probing and Manipulating the Lateral Pressure Profile in Lipid Bilayers Using Membrane-Active Peptides-A Solid-StateInternational journal of molecular sciences · 2022Article
- Studying KcsA Channel Clustering Using Single Channel Voltage-Clamp Fluorescence Imaging.Frontiers in physiology · 2022Article
- Spatiotemporal dynamics of PIEZO1 localization controls keratinocyte migration during wound healing.eLife · 2021Article
- Article
- Production of perdeuterated fucose from glyco-engineered bacteria.Glycobiology · 2021Article
- Modulation of Function, Structure and Clustering of KInternational journal of molecular sciences · 2020Review
- Clusters of cooperative ion channels enable a membrane-potential-based mechanism for short-term memory.eLife · 2020Article
- A Rationale for Mesoscopic Domain Formation in Biomembranes.Biomolecules · 2018Article
- Lipid bilayer mediates ion-channel cooperativity in a model of hair-cell mechanotransduction.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Protein crowding and lipid complexity influence the nanoscale dynamic organization of ion channels in cell membranes.Scientific reports · 2017Article
- Dash-and-Recruit Mechanism Drives Membrane Curvature Recognition by the Small Bacterial Protein SpoVM.Cell systems · 2017Article
- Shear Stress Regulates TRPV4 Channel Clustering and Translocation from Adherens Junctions to the Basal Membrane.Scientific reports · 2017Article
- Dynamics of Escherichia coli's passive response to a sudden decrease in external osmolarity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
17 authors.
Funding
Abstract
Mechanosensitive channels allow bacteria to respond to osmotic stress by opening a nanometer-sized pore in the cellular membrane. Although the underlying mechanism has been thoroughly studied on the basis of individual channels, the behavior of channel ensembles has yet to be elucidated. This work reveals that mechanosensitive channels of large conductance (MscL) exhibit a tendency to spatially cluster, and demonstrates the functional relevance of clustering. We evaluated the spatial distribution of channels in a lipid bilayer using patch-clamp electrophysiology, fluorescence and atomic force microscopy, and neutron scattering and reflection techniques, coupled with mathematical modeling of the mechanics of a membrane crowded with proteins. The results indicate that MscL forms clusters under a wide range of conditions. MscL is closely packed within each cluster but is still active and mechanosensitive. However, the channel activity is modulated by the presence of neighboring proteins, indicating membrane-mediated protein-protein interactions. Collectively, these results suggest that MscL self-assembly into channel clusters plays an osmoregulatory functional role in the membrane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.