ArticleJournal of hepatology2011
Alpha-fetoprotein-thymidine kinase-luciferase knockin mice: a novel model for dual modality longitudinal imaging of tumorigenesis in liver.
Article in Journal of hepatology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Constitutive AMPK activation prevents hepatocellular carcinoma development through inhibition of HNF4α activity.Science advances · 2026Article
- AFP promotes HCC progression by suppressing the HuR-mediated Fas/FADD apoptotic pathway.Cell death & disease · 2020Article
- Non-invasive Bioluminescence Monitoring of Hepatocellular Carcinoma Therapy in an HCR Mouse Model.Frontiers in oncology · 2019Article
- Transgenic Animal Models to Visualize Cancer-Related Cellular Processes by Bioluminescence Imaging.Frontiers in pharmacology · 2019Review
- Mice lacking RAP1 show early onset and higher rates of DEN-induced hepatocellular carcinomas in female mice.PloS one · 2018Article
- Alpha-fetoprotein-targeted reporter gene expression imaging in hepatocellular carcinoma.World journal of gastroenterology · 2016Review
- PET imaging of oncolytic VSV expressing the mutant HSV-1 thymidine kinase transgene in a preclinical HCC rat model.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- Illuminating cancer systems with genetically engineered mouse models and coupled luciferase reporters in vivo.Cancer discovery · 2013Review
- Comparative histomorphological review of rat and human hepatocellular proliferative lesions.Journal of toxicologic pathology · 2012Article
- Overexpression of a novel osteopetrosis-related gene CCDC154 suppresses cell proliferation by inducing G2/M arrest.Cell cycle (Georgetown, Tex.) · 2012Article
- Circulating tumor cells measurements in hepatocellular carcinoma.International journal of hepatology · 2012Article
- Radio-deoxynucleoside Analogs used for Imaging tk Expression in a Transgenic Mouse Model of Induced Hepatocellular Carcinoma.Theranostics · 2012Article
Corrections and comments
- Commented on byFocus.2011
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
BACKGROUND &
aimsHepatocellular carcinoma (HCC) is frequently a lethal disease and one of the few malignancies that is still increasing in incidence around the world. Better animal models are highly desired to investigate the molecular basis of HCC and to develop novel therapeutic strategies. Alpha-fetoprotein (Afp) gene is expressed in fetal liver, silenced soon after birth, and highly re-expressed in hepatocellular carcinomas (HCC). We aimed to take advantage of the dramatic re-expression of the Afp gene in HCC to develop a hepatocarcinogenesis reporter (HCR) mouse model for dual-modality, longitudinal in vivo imaging of liver tumor development, and progression.
methodsKnock in mice were established by placing a thymidinekinase (tk)-luciferase (luc) reporter gene cassette under the transcriptional control of the endogenous Afp promoter. DEN, a liver carcinogen, was used to induce liver tumors, which was monitored by both luc-based bioluminescent (BL) and tk-based positron emission tomography (PET) imaging.
resultsThe expression profile of luc was identical to that of the endogenous Afp gene during development. As early as 2 months after the exposure to DEN, BLI revealed multifocal signals in the liver, long before the appearance of histologically apparent neoplastic lesions. By 6 months, BL and PET dual imaging showed strong signals in malignant HCC. By serendipity, a strong BL signal was also detected in adult testes, a previously unknown site of Afp expression.
conclusionsThe HCR model enables longitudinal monitoring of liver tumor development and progression, providing a powerful tool in developing chemoprevention and therapeutic strategies for HCC.
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