ArticleJournal of neuroscience methods2011
A systematic evaluation of Schwann cell injection into acellular cold-preserved nerve grafts.
Article in Journal of neuroscience methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Mature Schwann Cells Derived From Human Induced Pluripotent Stem Cells Promote Peripheral Nerve Regeneration In Vivo.Neuroscience insights · 2026Article
- Adipose-derived stem cells in diabetic foot care: Bridging clinical trials and practical application.World journal of diabetes · 2024Review
- Schwann cells acquire a repair phenotype after assembling into spheroids and show enhanced in vivo therapeutic potential for promoting peripheral nerve repair.Bioengineering & translational medicine · 2024Article
- Nerve regeneration using the Bio 3D nerve conduit fabricated with spheroids.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2022Review
- Dynamic seeding versus microinjection of mesenchymal stem cells for acellular nerve allograft: an in vitro comparison.Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2022Article
- Article
- Introducing human adipose-derived mesenchymal stem cells to AvanceJournal of plastic, reconstructive & aesthetic surgery : JPRAS · 2020Article
- Pro-angiogenic scaffold-free Bio three-dimensional conduit developed from human induced pluripotent stem cell-derived mesenchymal stem cells promotes peripheral nerve regeneration.Scientific reports · 2020Article
- Advances in the repair of segmental nerve injuries and trends in reconstruction.Muscle & nerve · 2020Review
- Adhesion, distribution, and migration of differentiated and undifferentiated mesenchymal stem cells (MSCs) seeded on nerve allografts.Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2020Article
- Review
- A Novel Experimental Model to Determine the Axon-Promoting Effects of Grafted Cells After Peripheral Nerve Injury.Frontiers in cellular neuroscience · 2019Article
- Modern Trends for Peripheral Nerve Repair and Regeneration: Beyond the Hollow Nerve Guidance Conduit.Frontiers in bioengineering and biotechnology · 2019Review
- A Simple Dynamic Strategy to Deliver Stem Cells to Decellularized Nerve Allografts.Plastic and reconstructive surgery · 2018Article
- Introduction of neurosupportive cells into processed acellular nerve allografts results in greater number and more even distribution when injected compared to soaking techniques.Neurological research · 2017Article
- A Nerve Conduit Containing a Vascular Bundle and Implanted With Bone Marrow Stromal Cells and Decellularized Allogenic Nerve Matrix.Cell transplantation · 2017Article
- Article
- Finely Tuned Temporal and Spatial Delivery of GDNF Promotes Enhanced Nerve Regeneration in a Long Nerve Defect Model.Tissue engineering. Part A · 2015Article
- Repair, protection and regeneration of peripheral nerve injury.Neural regeneration research · 2015Article
- An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering.Journal of anatomy · 2015Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Peripheral nerve regeneration after injury depends on environmental cues and trophic support. Schwann cells (SCs) secrete trophic factors that promote neuronal survival and help guide axons during regeneration. The addition of SCs to acellular nerve grafts is a promising strategy for enhancing peripheral nerve regeneration; however, inconsistencies in seeding parameters have led to varying results. The current work sought to establish a systematic approach to seeding SCs in cold-preserved acellular nerve grafts. Studies were undertaken to (1) determine the needle gauge for optimal cell survival and minimal epineurial disruption during injection, (2) track the seeded SCs using a commercially available dye, and (3) evaluate the seeding efficiency of SCs in nerve grafts. It was determined that seeding with a 27-gauge needle resulted in the highest viability of SCs with the least damage to the epineurium. In addition, Qtracker(®) dye, a commercially available quantum dot nanocrystal, was used to label SCs prior to transplantation, which allowed visualization of the seeded SCs in nerve grafts. Finally, stereological methods were used to evaluate the seeding efficiency of SCs in nerve grafts immediately after injection and following a 1- or 3-day in vitro incubation in SC growth media. Using a systematic approach, the best needle gauge and a suitable dye for SC visualization in acellular nerve grafts were identified. Seeding efficiency in these grafts was also determined. The findings will lead to improvements ability to assess injection of cells (including SCs) for use with acellular nerve grafts to promote nerve regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.