ArticleThe Journal of molecular diagnostics : JMD2011
Custom design of a GeXP multiplexed assay used to assess expression profiles of inflammatory gene targets in normal colon, polyp, and tumor tissue.
Article in The Journal of molecular diagnostics : JMD, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- ELR positive CXCL chemokines are highly expressed in an animal model of ulcerative colitis.Journal of inflammation research · 2019Article
- Simultaneous detection of eight immunosuppressive chicken viruses using a GeXP analyser-based multiplex PCR assay.Virology journal · 2015Article
- GeXP analyzer-based multiplex reverse-transcription PCR assay for the simultaneous detection and differentiation of eleven duck viruses.BMC microbiology · 2015Article
- An advanced fragment analysis-based individualized subtype classification of pediatric acute lymphoblastic leukemia.Scientific reports · 2015Article
- Molecular Profiling of Multiplexed Gene Markers to Assess Viability of Ex Vivo Human Colon Explant Cultures.BioResearch open access · 2015Article
- Molecular Diagnostic Applications in Colorectal Cancer.Microarrays (Basel, Switzerland) · 2014Review
- A UDP-Glucose:Monoterpenol Glucosyltransferase Adds to the Chemical Diversity of the Grapevine Metabolome.Plant physiology · 2014Article
- Predictive gene signatures: molecular markers distinguishing colon adenomatous polyp and carcinoma.PloS one · 2014Article
- Gene expression signatures of energetic acclimatisation in the reef building coral Acropora millepora.PloS one · 2013Article
- Cellular defense system gene expression profiling of human whole blood: opportunities to predict health benefits in response to diet.Advances in nutrition (Bethesda, Md.) · 2012Review
- High-fat diet alters gene expression in the liver and colon: links to increased development of aberrant crypt foci.Digestive diseases and sciences · 2012Article
- Novel multiplex method to assess insulin, leptin and adiponectin regulation of inflammatory cytokines associated with colon cancer.Molecular biology reports · 2012Article
- Janice Drew's work on diet and cancer.World journal of gastrointestinal pathophysiology · 2011Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Colon cancers are characterized by aberrant gene expression signatures associated with disease initiation and progression. Identification of aberrant gene expression associated with colon carcinogenesis has increased significantly with application of gene array technologies. Downstream processing of these data has been hindered by the lack of robust multiplexed gene quantitative technologies facilitating study of the identified multiple gene targets. The GenomeLab Genetic Analysis System presents a novel technology platform for quantitative multiplexed gene expression analysis. This report describes the custom design of a GeXP multiplexed assay used to assess expression profiles of 14 inflammatory gene targets in normal, polyp, and tumor tissue. Characteristic normal, polyp, and tumor tissue gene expression profiles were obtained. Statistical analysis confirmed comparable relative quantitation of gene expression using the GeXP, macroarray, and single-plex real-time polymerase chain reaction assays. GeXP assays may be usefully applied in clinical and regulatory studies of multiple gene targets. This system permits custom-design options for relative quantification of multiple gene target expression, simultaneously in a single reaction, using nanogram quantities of total RNA template. The system provides an approach to advance the study of multiple targets identified from gene array analysis with potential for characterizing gene expression signatures in clinical diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.