ArticleStem cells and development2011
Isolation and characterization of node/notochord-like cells from mouse embryonic stem cells.
Article in Stem cells and development, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- The notochord: development, disease and stem cell-based modelling.Development (Cambridge, England) · 2026Review
- Timely TGFβ signalling inhibition induces notochord.Nature · 2025Article
- In vitro modelling of anterior primitive streak patterning with human pluripotent stem cells identifies the path to notochord progenitors.Development (Cambridge, England) · 2024Article
- Article
- Review
- A 37 kb region upstream of brachyury comprising a notochord enhancer is essential for notochord and tail development.Development (Cambridge, England) · 2021Article
- A Multiplexed Barcodelet Single-Cell RNA-Seq Approach Elucidates Combinatorial Signaling Pathways that Drive ESC Differentiation.Cell stem cell · 2020Article
- Article
- Human iPSCs can be differentiated into notochordal cells that reduce intervertebral disc degeneration in a porcine model.Theranostics · 2019Article
- Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells.Stem cell research & therapy · 2018Article
- Notochord morphogenesis in mice: Current understanding & open questions.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Review
- Brachyury and SMAD signalling collaboratively orchestrate distinct mesoderm and endoderm gene regulatory networks in differentiating human embryonic stem cells.Development (Cambridge, England) · 2015Article
- Modulating notochordal differentiation of human induced pluripotent stem cells using natural nucleus pulposus tissue matrix.PloS one · 2014Article
- Notochordal cell-derived therapeutic strategies for discogenic back pain.Global spine journal · 2013Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The homeobox gene Noto is expressed in the node and its derivative the notochord. Here we use a targeted Noto-GFP reporter to isolate and characterize node/notochord-like cells derived from mouse embryonic stem cells. We find very few Noto-expressing cells after spontaneous differentiation. However, the number of Noto-expressing cells was increased when using Activin A to induce a Foxa2- and Brachyury-expressing progenitor population, whose further differentiation into Noto-expressing cells was improved by simultaneous inhibition of BMP, Wnt, and retinoic acid signaling. Noto-GFP(+) cells expressed the node/notochord markers Noto, Foxa2, Shh, Noggin, Chordin, Foxj1, and Brachyury; showed a vacuolarization characteristic of notochord cells; and can integrate into midline structures when grafted into Hensen's node of gastrulating chicken embryos. The ability to generate node/notochord-like cells in vitro will aid the biochemical characterization of these developmentally important structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.