ArticleACS nano2011
Probing intracellular biomarkers and mediators of cell activation using nanosensors and bioorthogonal chemistry.
Article in ACS nano, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Bioimaging Probes Based on Magneto-Fluorescent Nanoparticles.Pharmaceutics · 2023Review
- Bioorthogonal Click of Colloidal Gold Nanoparticles to Antibodies In vivo.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Phasor Analysis of Fluorescence Lifetime Enables Quantitative Multiplexed Molecular Imaging of Three Probes.Analytical chemistry · 2022Article
- Nanoparticles and bioorthogonal chemistry joining forces for improved biomedical applications.Nanoscale advances · 2021Review
- Engineering Prodrug Nanomedicine for Cancer Immunotherapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Review
- Magnetic Nanoparticles in Cancer Therapy and Diagnosis.Advanced healthcare materials · 2020Review
- Article
- Pushing the limits of detection for proteins secreted from single cells using quantum dots.The Analyst · 2019Article
- Click Chemistry-Mediated Nanosensors for Biochemical Assays.Theranostics · 2016Review
- Recent advances in covalent, site-specific protein immobilization.F1000Research · 2016Review
- Recent Developments in Magnetic Diagnostic Systems.Chemical reviews · 2015Review
- Enhancing reactivity for bioorthogonal pretargeting by unmasking antibody-conjugated trans-cyclooctenes.Bioconjugate chemistry · 2015Article
- Molecular extraction in single live cells by sneaking in and out magnetic nanomaterials.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- The inverse electron demand Diels-Alder click reaction in radiochemistry.Journal of labelled compounds & radiopharmaceuticals · 2014Review
- Microfluidic on-chip capture-cycloaddition reaction to reversibly immobilize small molecules or multi-component structures for biosensor applications.Journal of visualized experiments : JoVE · 2013Article
- Multimodal iron oxide nanoparticles for hybrid biomedical imaging.NMR in biomedicine · 2013Review
- On-chip bioorthogonal chemistry enables immobilization of in situ modified nanoparticles and small molecules for label-free monitoring of protein binding and reaction kinetics.Lab on a chip · 2012Article
- Magnetic nanoparticles in magnetic resonance imaging and diagnostics.Pharmaceutical research · 2012Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Nanomaterials offer unique physical properties that make them ideal biosensors for scant cell populations. However, specific targeting of nanoparticles to intracellular proteins has been challenging. Here, we describe a technique to improve intracellular biomarker sensing using nanoparticles that is based on bioorthogonal chemistry. Using trans-cyclooctene-modified affinity ligands that are administered to semipermeabilized cells and revealed by cycloaddition reaction with tetrazine-conjugated nanoparticles, we demonstrate site-specific amplification of nanomaterial binding. We also show that this technique is capable of sensing protein biomarkers and phosho-protein signal mediators, both within the cytosol and nucleus, via magnetic or fluorescent modalities. We expect the described method will have broad applications in nanomaterial-based diagnostics and therapeutics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.