ArticlePlant cell reports2011
Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 86 citations in OpenAlex.
- Retraction Note: Use of the cryptogein gene to stimulate the accumulation of bacopa saponins in transgenic Bacopa monnieri plants.Plant cell reports · 2024Article
- Enhanced production of Bacopa saponins by repeated batch strategy in bioreactor.Bioprocess and biosystems engineering · 2022Article
- Biotechnology for propagation and secondary metabolite production in Bacopa monnieri.Applied microbiology and biotechnology · 2022Review
- Biotechnological production of bacosides from cell and organ cultures of Bacopa monnieri.Applied microbiology and biotechnology · 2022Review
- Spontaneous Regeneration of Plantlets Derived from Hairy Root Cultures ofPlants (Basel, Switzerland) · 2022Article
- Highly efficient hairy root genetic transformation and applications in citrus.Frontiers in plant science · 2022Article
- Bacopasaponins with cytotoxic activity against human breast cancer cells in vitro.Molecular biology reports · 2021Article
- Hairy root culture technology: applications, constraints and prospect.Applied microbiology and biotechnology · 2021Review
- Statistical optimization of bacoside A biosynthesis in plant cell suspension cultures using response surface methodology.3 Biotech · 2020Article
- Plant triterpenoid saponins: biosynthesis, in vitro production, and pharmacological relevance.Protoplasma · 2019Review
- Optimizing culture conditions for establishment of hairy root culture of Semecarpus anacardium L.3 Biotech · 2017Article
- Article
- Analysis of propagation of Bacopa monnieri (L.) from hairy roots, elicitation and Bacoside A contents of Ri transformed plants.World journal of microbiology & biotechnology · 2016Article
- Induction of hairy roots byScientia horticulturae · 2016Article
- Genetic engineering and sustainable production of ornamentals: current status and future directions.Plant cell reports · 2012Review
- Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures.Plant cell reports · 2012Article
- Plant biotechnology in support of the Millennium Goals II.Plant cell reports · 2011Article
Corrections and comments
- Retraction · 2024-09-25Concerns/Issues about Image · Duplication of/in Image · Euphemisms for Duplication · Investigation by Journal/Publisher · Objections by Author(s) · Unreliable Results and/or Conclusions ·
- Retracted
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed an efficient transformation system for Bacopa monnieri, an important Indian medicinal plant, using Agrobacterium rhizogenes strains LBA 9402 and A4. Transformed roots induced by strain LBA 9402 spontaneously dedifferentiated to callus while excised roots induced by strain A4 spontaneously showed induction of shoot buds within 10 days. PCR and RT-PCR analysis revealed the presence and expression of the rolAB and rolC genes at the transcription level in pRi A4 transformed cultures indicating that the TL-DNA was integrated retained and expressed in the A4-Ri transformed shoots. Transformed calli showed the presence of rolAB or rol A, TR and ags genes. Transformed plants showed morphological features typically seen in transgenic plants produced by A. rhizogenes. Growth and biomass accumulation was significantly higher in the transformed shoots (twofold) and roots (fourfold) than in the non-transformed (WT) plants. In pRi A4-transformed plants, the content of bacopasaponin D, bacopasaponin F, bacopaside II and bacopaside V was enhanced significantly as compared to WT plants of similar age while bacoside A3 and bacopasaponin C content was comparable with that of WT plants. Significant increase in content of five bacopa saponins could be detected in pRi 9402-transformed callus cultures. There is an overall stimulatory effect on accumulation of bacopa saponins in transformed plants and cells of B. monnieri establishing the role of endogenous elicitation by Ri T-DNA of A. rhizogenes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.