ArticleCardiovascular psychiatry and neurology2011
Slice cultures as a model to study neurovascular coupling and blood brain barrier in vitro.
Article in Cardiovascular psychiatry and neurology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- From Organotypic Mouse Brain Slices to Human Alzheimer's Plasma Biomarkers: A Focus on Nerve Fiber Outgrowth.Biomolecules · 2024Article
- Organotypic brain slices as a model to study the neurotropism of the highly pathogenic Nipah and Ebola viruses.The Journal of general virology · 2024Article
- Intraventricular haemorrhage in premature infants: the role of immature neuronal salt and water transport.Brain : a journal of neurology · 2024Article
- Article
- Cortical Oxygenation during a Motor Task to Evaluate Recovery in Subacute Stroke Patients: A Study with Near-Infrared Spectroscopy.Neurology international · 2022Article
- The neurovascular unit in leukodystrophies: towards solving the puzzle.Fluids and barriers of the CNS · 2022Review
- Preparation and Culture of Organotypic Hippocampal Slices for the Analysis of Brain Metastasis and Primary Brain Tumor Growth.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Hallmarks of NLRP3 inflammasome activation are observed in organotypic hippocampal slice culture.Immunology · 2020Article
- Genetically Encoded Tools for Research of Cell Signaling and Metabolism under Brain Hypoxia.Antioxidants (Basel, Switzerland) · 2020Review
- Neuroprotection by Propofol Post-Conditioning: Focus on PKMζ/KCC2 Pathway Activity.Cellular and molecular neurobiology · 2018Article
- Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2018Article
- Long-Term Culture of Organotypic Hippocampal Slice from Old 3xTg-AD Mouse: AnPsychiatry investigation · 2018Article
- Bioenergetic Mechanisms of Seizure Control.Frontiers in cellular neuroscience · 2018Review
- Extensive astrocyte synchronization advances neuronal coupling in slow wave activity in vivo.Scientific reports · 2017Article
- Organotypic brain slice cultures: A review.Neuroscience · 2015Review
- Spreading depression transiently disrupts myelin via interferon-gamma signaling.Experimental neurology · 2015Article
- The janus facet of nanomaterials.BioMed research international · 2015Review
- Article
- IFNγ-stimulated dendritic cell exosomes as a potential therapeutic for remyelination.Journal of neuroimmunology · 2014Article
- Blood-brain barrier structure and function and the challenges for CNS drug delivery.Journal of inherited metabolic disease · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proper neuronal functioning depends on a strictly regulated interstitial environment and tight coupling of neuronal and metabolic activity involving adequate vascular responses. These functions take place at the blood brain barrier (BBB) composed of endothelial cells, basal lamina covered with pericytes, and the endfeet of perivascular astrocytes. In conventional in vitro models of the BBB, some of these components are missing. Here we describe a new model system for studying BBB and neurovascular coupling by using confocal microscopy and fluorescence staining protocols in organotypic hippocampal slice cultures. An elaborated network of vessels is retained in culture in spite of the absence of blood flow. Application of calcein-AM either from the interstitial or from the luminal side resulted in different staining patterns indicating the maintenance of a barrier. By contrast, the ethidium derivative MitoSox penetrated perivascular basal lamina and revealed free radical formation in contractile cells embracing the vessels, likely pericytes.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.