ArticleJournal of medical microbiology2011
Pneumococcal DNA is not detectable in the blood of healthy carrier children by real-time PCR targeting the lytA gene.
Article in Journal of medical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Clinical manifestations, serotype distribution, and incidence of pediatric invasive pneumococcal disease in Catalonia (Spain), 2018-2022.European journal of pediatrics · 2025Observational
- Rapid diagnostic value of next-generation sequencing-based technologies in childhood pneumonia.Frontiers in pediatrics · 2025Article
- The drop in reported invasive pneumococcal disease among adults during the first COVID-19 wave in the Netherlands explained.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2021Article
- Pediatric Community-Acquired Pneumonia in the United States: Changing Epidemiology, Diagnostic and Therapeutic Challenges, and Areas for Future Research.Infectious disease clinics of North America · 2018Review
- Detection of Pneumococcal DNA in Blood by Polymerase Chain Reaction for Diagnosing Pneumococcal Pneumonia in Young Children From Low- and Middle-Income Countries.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
- Evaluation of Pneumococcal Load in Blood by Polymerase Chain Reaction for the Diagnosis of Pneumococcal Pneumonia in Young Children in the PERCH Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
- Evaluation of chest radiography, lytA real-time PCR, and other routine tests for diagnosis of community-acquired pneumonia and estimation of possible attributable fraction of pneumococcus in northern Togo.Epidemiology and infection · 2017Article
- Burden of Pneumococcal Disease in Northern Togo before the Introduction of Pneumococcal Conjugate Vaccine.PloS one · 2017Article
- Epidemiology of Acute Lower Respiratory Tract Infection in HIV-Exposed Uninfected Infants.Pediatrics · 2016Article
- Enhanced Diagnosis of Pneumococcal Bacteremia Using Antigen- and Molecular-Based Tools on Blood Specimens in Mali and Thailand: A Prospective Surveillance Study.The American journal of tropical medicine and hygiene · 2016Article
- Use of Blood Smears and Dried Blood Spots for Polymerase Chain Reaction-Based Detection and Quantification of Bacterial Infection and Plasmodium falciparum in Severely Ill Febrile African Children.The American journal of tropical medicine and hygiene · 2016Article
- How recent advances in molecular tests could impact the diagnosis of pneumonia.Expert review of molecular diagnostics · 2016Review
- Severe Acute Respiratory Illness Deaths in Sub-Saharan Africa and the Role of Influenza: A Case Series From 8 Countries.The Journal of infectious diseases · 2015Article
- Human metapneumovirus-associated severe acute respiratory illness hospitalisation in HIV-infected and HIV-uninfected South African children and adults.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2015Article
- Article
- Standardisation and evaluation of a quantitative multiplex real-time PCR assay for the rapid identification ofPneumonia (Nathan Qld.) · 2015Article
- Biomarkers for community-acquired pneumonia in the emergency department.Current infectious disease reports · 2014Article
- Diagnostic value of serum pneumococcal DNA load during invasive pneumococcal infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Article
- Diagnosis of pneumococcal pneumonia: current pitfalls and the way forward.Infection & chemotherapy · 2013Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diagnosis of invasive pneumococcal disease (IPD) is currently based on culture methods, which lack sensitivity, especially after antibiotic therapy. Molecular methods have improved sensitivity and do not require viable bacteria; however, their use is complicated by reports of low specificity with some assays. The present study investigated the specificity of a real-time PCR targeting lytA for the detection of IPD. A group of 147 healthy children, aged 6 months to 16 years (mean 6.4 years, median 4.9 years, interquartile range 6.4 years), who were in hospital for routine examinations, were tested for pneumococcal carrier status and for the presence of detectable pneumococcal DNA in their blood by real-time PCR targeting the pneumococcal lytA gene. In addition, 35 culture-positive biological samples were analysed. Urine was examined for the presence of pneumococcal DNA and C-polysaccharide antigen. Carriage was detected in 77 of the 147 subjects (52.4 %); however, regardless of carrier status, none of the subjects had a positive result from blood. Analysis of the culture-positive biological samples yielded positive results in 100 % (15/15) of cerebrospinal fluid samples and 95 % (19/20) of blood samples. All urine samples from healthy carriers were negative for DNA, whilst antigenuria was detected in 44/77 carriers (57.1 %). In conclusion, real-time PCR is both sensitive and specific and can be a useful tool in the routine diagnosis of IPD. Its sensitivity, which surpasses that of other methods for this purpose, does not come at the cost of reduced specificity.
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