ArticleBMC microbiology2011
Multilocus variable-number tandem-repeat analysis, pulsed-field gel electrophoresis, and antimicrobial susceptibility patterns in discrimination of sporadic and outbreak-related strains of Yersinia enterocolitica.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- In-host evolution of Yersinia enterocolitica during a chronic human infection.Nature communications · 2025Article
- Comparison of Multiple-Locus Variable-Number Tandem Repeat Analysis Profiles of EnteropathogenicAnimals : an open access journal from MDPI · 2023Article
- Controlled Experimental Infection in Pigs with a Strain of Yersinia enterocolitica Harboring Genetic Markers for Human Pathogenicity: Colonization and Stability.Infection and immunity · 2023Article
- First Description of a Yersinia pseudotuberculosis Clonal Outbreak in France, Confirmed Using a New Core Genome Multilocus Sequence Typing Method.Microbiology spectrum · 2022Article
- Prudent Antimicrobial Use Is Essential to Prevent the Emergence of Antimicrobial Resistance inFrontiers in microbiology · 2022Article
- Diversity of Yersinia enterocolitica isolated from pigs in a French slaughterhouse over 2 years.MicrobiologyOpen · 2019Article
- Evaluating sub-typing methods for pathogenic Yersinia enterocolitica to support outbreak investigations in New Zealand.Epidemiology and infection · 2019Article
- Capillary electrophoresis applied to DNA: determining and harnessing sequence and structure to advance bioanalyses (2009-2014).Analytical and bioanalytical chemistry · 2015Review
- An outbreak of Yersinia enterocolitica in a captive colony of African green monkeys (Chlorocebus aethiops sabaeus) in the Caribbean.Comparative medicine · 2013Article
- Multiple-locus variable-number tandem-repeat analysis in genotyping Yersinia enterocolitica strains from human and porcine origins.Journal of clinical microbiology · 2013Article
- Characterization of Yersinia enterocolitica biotype 1A strains isolated from swine slaughterhouses and markets.TheScientificWorldJournal · 2013Article
- Yersinia enterocolitica strains associated with human infections in Switzerland 2001-2010.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Piglets are a source of pathogenic Yersinia enterocolitica on fattening-pig farms.Applied and environmental microbiology · 2012Article
- Multiple-locus variable-number tandem-repeat analysis of pathogenic Yersinia enterocolitica in China.PloS one · 2012Article
- Pathogenesis of Y. enterocolitica and Y. pseudotuberculosis in Human Yersiniosis.Journal of pathogens · 2011Article
- Antimicrobial resistance and plasmid replicons in Yersinia enterocolitica strains isolated in Brazil in 30 years.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe assessed the potential of multilocus variable-number tandem-repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing for discriminating 104 sporadic and outbreak-related Yersinia enterocolitica (YE) bio/serotype 3-4/O:3 and 2/O:9 isolates. MLVA using six VNTR markers was performed in two separate multiplex PCRs, and the fluorescently labeled PCR products were accurately sized on an automated DNA sequencer.
resultsMLVA discriminated 82 sporadic YE 3-4/O:3 and 2/O:9 strains into 77 types, whereas PFGE with the restriction enzyme NotI discriminated the strains into 23 different PFGE pulsotypes. The discriminatory index for a sporadic strain was 0.862 for PFGE and 0.999 for MLVA. MLVA confirmed that a foodborne outbreak in the city of Kotka, Finland in 2003 had been caused by a multiresistant YE 4/O:3 strain that was distinctly different from those of epidemiologically unrelated strains with an identical PFGE pulsotype. The multiresistance of Y. enterocolitica strains (19% of the sporadic strains) correlated significantly (p = 0.002) with travel abroad. All of the multiresistant Y. enterocolitica strains belonged to four PFGE pulsotypes that did not contain any susceptible strains. Resistance to nalidixic acid was related to changes in codons 83 or 87 that stemmed from mutations in the gyrA gene. The conjugation experiments demonstrated that resistance to CHL, STR, and SUL was carried by a conjugative plasmid.
conclusionsMLVA using six loci had better discriminatory power than PFGE with the NotI enzyme. MLVA was also a less labor-intensive method than PFGE and the results were easier to analyze. The conjugation experiments demonstrated that a resistance plasmid can easily be transferred between Y. enterocolitica strains. Antimicrobial multiresistance of Y. enterocolitica strains was significantly associated with travel abroad.
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