ArticlePlant methods2011
A simple and efficient method for isolating small RNAs from different plant species.
Article in Plant methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Characterization and transcriptomic analysis provide novel insights into the sexual differentiation of the dioecious plant Bama Huoma (Cannabis sativa).BMC plant biology · 2025Article
- Plant-induced bacterial gene silencing: a novel control method for bacterial wilt disease.Frontiers in plant science · 2024Article
- Strategies and Best Practice in Cloning Small RNAs.Gene technology · 2020Article
- Delineating effect of corn microRNAs and matrix, ingested as whole food, on gut microbiota in a rodent model.Food science & nutrition · 2020Article
- ThePlant physiology · 2020Article
- Wisteria Vein Mosaic Virus Detected for the First Time in Iran from an Unknown Host by Analysis of Aphid Vectors.The plant pathology journal · 2020Article
- Extensive Degradation and Low Bioavailability of Orally Consumed Corn miRNAs in Mice.Nutrients · 2018Article
- Article
- MicroRNA profiling provides insights into post-transcriptional regulation of gene expression in chickpea root apex under salinity and water deficiency.Scientific reports · 2017Article
- Article
- De novo transcriptome assembly ofGenomics data · 2016Article
- Deep-sequence profiling of miRNAs and their target prediction in Monotropa hypopitys.Plant molecular biology · 2016Article
- Salt Induces Features of a Dormancy-Like State in Seeds of Eutrema (Thellungiella) salsugineum, a Halophytic Relative of Arabidopsis.Frontiers in plant science · 2016Article
- Article
- An efficient method for miRNA detection and localization in crop plants.Frontiers in plant science · 2015Article
- Rapid and efficient isolation of high-quality small RNAs from recalcitrant plant species rich in polyphenols and polysaccharides.PloS one · 2014Article
- Transcriptome profiling of fruit development and maturation in Chinese white pear (Pyrus bretschneideri Rehd).BMC genomics · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSmall RNAs emerged over the last decade as key regulators in diverse biological processes in eukaryotic organisms. To identify and study small RNAs, good and efficient protocols are necessary to isolate them, which sometimes may be challenging due to the composition of specific tissues of certain plant species. Here we describe a simple and efficient method to isolate small RNAs from different plant species.
resultsWe developed a simple and efficient method to isolate small RNAs from different plant species by first comparing different total RNA extraction protocols, followed by streamlining the best one, finally resulting in a small RNA extraction method that has no need of first total RNA extraction and is not based on the commercially available TRIzol® Reagent or columns. This small RNA extraction method not only works well for plant tissues with high polysaccharide content, like cactus, agave, banana, and tomato, but also for plant species like Arabidopsis or tobacco. Furthermore, the obtained small RNA samples were successfully used in northern blot assays.
conclusionHere we provide a simple and efficient method to isolate small RNAs from different plant species, such as cactus, agave, banana, tomato, Arabidopsis, and tobacco, and the small RNAs from this simplified and low cost method is suitable for downstream handling like northern blot assays.
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