ArticleJournal of translational medicine2011
CXC receptor-4 mRNA silencing abrogates CXCL12-induced migration of colorectal cancer cells.
Article in Journal of translational medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Trial
- CXCR4, CXCL12 and the relative CXCL12-CXCR4 expression as prognostic factors in colon cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Trial
- Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3DFrontiers in drug delivery · 2023Article
- Metformin counteracts stimulatory effects induced by insulin in primary breast cancer cells.Journal of translational medicine · 2022Article
- Toxicity Profiling of Bacterial Inclusion Bodies in Human Caco-2 Cells.Frontiers in bioengineering and biotechnology · 2022Article
- CXCL12-mediated HOXB5 overexpression facilitates Colorectal Cancer metastasis through transactivating CXCR4 and ITGB3.Theranostics · 2021Article
- The CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling.Cell communication and signaling : CCS · 2018Article
- Expression of CXCL12 and its receptor CXCR4 in patients with adenomyosis.Oncology letters · 2017Article
- A novel variant on chromosome 6p21.1 is associated with the risk of developing colorectal cancer: a two-stage case-control study in Han Chinese.BMC cancer · 2016Article
- The chemokines CCR1 and CCRL2 have a role in colorectal cancer liver metastasis.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- CXCL12/CXCR4 display an inverse mRNA expression profile in gastric carcinoma that correlates with tumor progression.Oncology letters · 2016Article
- Significantly inhibitory effects of low molecular weight heparin (Fraxiparine) on the motility of lung cancer cells and its related mechanism.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Critical appraisal of quantitative PCR results in colorectal cancer research: can we rely on published qPCR results?Molecular oncology · 2014Review
- The influence of lentivirus-mediated CXCR4 RNA interference on hepatic metastasis of colorectal cancer.International journal of oncology · 2014Article
- Effect of CXCL12/CXCR4 on increasing the metastatic potential of non-small cell lung cancerOncology letters · 2014Article
- Chemokine receptor CCR6 expression is regulated by miR-518a-5p in colorectal cancer cells.Journal of translational medicine · 2014Article
- The role of chemoattractant receptors in shaping the tumor microenvironment.BioMed research international · 2014Review
- The CXCL12/CXCR4/CXCR7 ligand-receptor system regulates neuro-glio-vascular interactions and vessel growth during human brain development.Journal of inherited metabolic disease · 2013Article
- The CXCL12/CXCR4 autocrine loop increases the metastatic potential of non-small cell lung cancer in vitro.Oncology letters · 2013Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInteractions between CXCR4 and its ligand CXCL12 have been shown to be involved in cancer progression in colorectal cancer (CRC). We performed a comparative CXCL12/CXCR4 expression analysis and assessed the effect of external CXCL12 stimulation on migration of CRC cells without and with CXCR4 inhibition.
methodsExpression of CXCL12/CXCR4 was assessed by quantitative real-time PCR, ELISA and immunohistochemistry in resection specimens of 50 CRC patients as well as in the corresponding normal tissues and in three human CRC cell lines with different metastatic potential (Caco-2, SW480 and HT-29). Migration assays were performed after stimulation with CXCL12 and CXCR4 was inhibited by siRNA and neutralizing antibodies.
resultsIn CRC tissues CXCL12 was significantly down-regulated and CXCR4 was significantly up-regulated compared to the corresponding normal tissues. In cell lines CXCR4 was predominantly expressed in SW480 and less pronounced in HT-29 cells. CXCL12 was only detectable in Caco-2 cells. CXCL12 stimulation had no impact on Caco-2 cells but significantly increased migration of CXCR4 bearing SW480 and HT-29 cells. This effect was significantly abrogated by neutralizing anti-CXCR4 antibody as well as by CXCR4 siRNAs (P < 0.05).
conclusionsCXCR4 expression was up-regulated in CRC and CXCL12 stimulation increased migration in CXCR4 bearing cell lines. Migration was inhibited by both neutralizing CXCR4 antibodies and CXCR4 siRNAs. Thus, the expression and functionality of CXCR4 might be associated with the metastatic potential of CRC cells and CXCL12/CXCR4 interactions might therefore constitute a promising target for specific treatment interventions.
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