Evidence map›Paper›PMID 21348869›Full record

ArticleJournal of microscopy2011

Rapid overlapping-volume acquisition and reconstruction (ROVAR): automated 3D tiling for high-resolution, large field-of-view optical microscopy.

J L Schroeder, M Bakalar, T J Pohida, R S Balaban

Abstract read
In one paragraph

Article in Journal of microscopy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
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  5. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

J L SchroederNHLBI, Laboratory of Cardiac Energetics, Maryland, U.S.A.
M Bakalar
T J Pohida
R S Balaban

Funding

NHLBI Animal Program DivisionZIGHL006020 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI CLARK, TANNIA · 2009 to 2025
$47.3M
Signal Processing And Instrumentation SectionZIACT000261 · CIT · CENTER FOR INFORMATION TECHNOLOGY · PI POHIDA, THOMAS J · 2009 to 2021
$16.7M
Intra-vital microscopy using non-linear optical techniquesZIAHL004610 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI BALABAN, ROBERT · 2009 to 2020
$10.9M
Intramural NIH HHS Z99 HL999999
6 · The paper itself

Abstract

Micrometer-scale three-dimensional data from fluorescence microscopes offer unique insight into cellular morphology and function by resolving subcellular locations of fluorescent dyes and proteins. To increase field-of-view size while using a high-resolution multiphoton microscope, we have created an automated system of rapidly acquiring overlapping image stacks from multiple fields-of-view along a nonplanar tissue surface. Each image stack is acquired only between the surface and the maximal penetrating depth, as determined by the image signal-to-background ratio. This results in the acquisition of the volume containing visible tissue along the tissue surface, excluding the empty volume above the tissue and the volume beyond the maximum imaging depth within the tissue. The automated collection of overlapping volumes is followed by reconstruction that can efficiently generate a single three-dimensional volume of the tissue surface. This approach yields data spanning multiple millimetres at micrometre resolution that is faster while requiring less work from the microscope operator. The advantages of the system are demonstrated by acquisition of data from intact, unfixed organs without a coverglass both in vivo and in situ.

Indexed as

AnimalsCytological TechniquesImaging, Three-DimensionalMiceMice, Inbred BALB CMicroscopy, FluorescencePathology

Identifiers

PMID21348869
PMCPMC3115409

What OpenQuestion holds

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LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.