ArticleAnnals of biomedical engineering2011
The effects of combined cyclic stretch and pressure on the aortic valve interstitial cell phenotype.
Article in Annals of biomedical engineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- In-Silico assessment of aortic valve function and mechanics under hypertension.Frontiers in cardiovascular medicine · 2025Article
- Strategies for Development of Synthetic Heart Valve Tissue Engineering Scaffolds.Progress in materials science · 2023Article
- Challenges of aortic valve tissue culture - maintenance of viability and extracellular matrix in the pulsatile dynamic microphysiological system.Journal of biological engineering · 2023Article
- The effects of strain history on aortic valve interstitial cell activation in a 3D hydrogel environment.APL bioengineering · 2023Article
- Aortic valve cell microenvironment: Considerations for developing a valve-on-chip.Biophysics reviews · 2021Review
- Crosstalk of Diabetic Conditions with Static Versus Dynamic Flow Environment-Impact on Aortic Valve Remodeling.International journal of molecular sciences · 2021Article
- Degeneration of Aortic Valves in a Bioreactor System with Pulsatile Flow.Biomedicines · 2021Article
- On the role of predicted in vivo mitral valve interstitial cell deformation on its biosynthetic behavior.Biomechanics and modeling in mechanobiology · 2021Article
- The Ryanodine Receptor Contributes to the Lysophosphatidylcholine-Induced Mineralization in Valvular Interstitial Cells.Cardiovascular engineering and technology · 2020Article
- The effect of physiological stretch and the valvular endothelium on mitral valve proteomes.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- The Three-Dimensional Microenvironment of the Mitral Valve: Insights into the Effects of Physiological Loads.Cellular and molecular bioengineering · 2018Article
- Regulation of valve interstitial cell homeostasis by mechanical deformation: implications for heart valve disease and surgical repair.Journal of the Royal Society, Interface · 2017Article
- Critical Role of Coaptive Strain in Aortic Valve Leaflet Homeostasis: Use of a Novel Flow Culture Bioreactor to Explore Heart Valve Mechanobiology.Journal of the American Heart Association · 2016Article
- Identification of side- and shear-dependent microRNAs regulating porcine aortic valve pathogenesis.Scientific reports · 2016Article
- Simulation of early calcific aortic valve disease in a 3D platform: A role for myofibroblast differentiation.Journal of molecular and cellular cardiology · 2016Article
- Mechanobiology in Cardiovascular Disease Management: Potential Strategies and Current Needs.Frontiers in bioengineering and biotechnology · 2016Article
- Integrating valve-inspired design features into poly(ethylene glycol) hydrogel scaffolds for heart valve tissue engineering.Acta biomaterialia · 2015Article
- The role of valvular endothelial cell paracrine signaling and matrix elasticity on valvular interstitial cell activation.Biomaterials · 2014Article
- Aortic valve: mechanical environment and mechanobiology.Annals of biomedical engineering · 2013Review
- Noggin attenuates the osteogenic activation of human valve interstitial cells in aortic valve sclerosis.Cardiovascular research · 2013Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Aortic valve interstitial cells (VIC) can exhibit phenotypic characteristics of fibroblasts, myofibroblasts, and smooth muscle cells. Others have proposed that valve cells become activated and exhibit myofibroblast or fibroblast characteristics during disease initiation and progression; however, the cues that modulate this phenotypic change remain unclear. We hypothesize that the mechanical forces experienced by the valve play a role in regulating the native phenotype of the valve and that altered mechanical forces result in an activated phenotype. Using a novel ex vivo cyclic stretch and pressure bioreactor, we subjected porcine aortic valve (AV) leaflets to combinations of normal and pathological stretch and pressure magnitudes. The myofibroblast markers α-SMA and Vimentin, along with the smooth muscle markers Calponin and Caldesmon, were analyzed using immunohistochemistry and immunoblotting. Tissue structure was analyzed using Movat's pentachrome staining. We report that pathological stretch and pressure inhibited the contractile and possibly myofibroblast phenotypes as indicated by downregulation of the proteins α-SMA, Vimentin, and Calponin. In particular, Calponin downregulation implies depolymerization of actin filaments and possible conversion to a more synthetic (non-contractile) phenotype. This agreed well with the increase in spongiosa and fibrosa thickness observed under elevated pressure and stretch that are typically indicative of increased matrix synthesis. Our study therefore demonstrates how cyclic stretch and pressure may possibly act together to modulate the AVIC phenotype.
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