ArticlePloS one2011
Intestinal epithelial stem/progenitor cells are controlled by mucosal afferent nerves.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 66 citations in OpenAlex.
- Vasoactive intestinal peptide promotes secretory differentiation and mitigates radiation-induced intestinal injury.Stem cell research & therapy · 2024Article
- Article
- The neuroscience of cancer.Nature · 2023Review
- Role of Muscarinic Acetylcholine Receptors in Intestinal Epithelial Homeostasis: Insights for the Treatment of Inflammatory Bowel Disease.International journal of molecular sciences · 2023Review
- Identification of a neural development gene expression signature in colon cancer stem cells reveals a role foriScience · 2022Article
- Muscarinic Receptors Associated with Cancer.Cancers · 2022Review
- From the intestinal mucosal barrier to the enteric neuromuscular compartment: an integrated overview on the morphological changes in Parkinson's disease.European journal of histochemistry : EJH · 2021Review
- Modeling Intestinal Stem Cell Function with Organoids.International journal of molecular sciences · 2021Review
- Muscarinic receptor M3 contributes to intestinal stem cell maintenance via EphB/ephrin-B signaling.Life science alliance · 2021Article
- Hair follicle stem cells as a skin-organizing signaling center during adult homeostasis.The EMBO journal · 2021Review
- Regulation of the Autonomic Nervous System on Intestine.Frontiers in physiology · 2021Review
- Serotonin as a Mitogen in the Gastrointestinal Tract: Revisiting a Familiar Molecule in a New Role.Cellular and molecular gastroenterology and hepatology · 2021Review
- Functional circuits and signal processing in the enteric nervous system.Cellular and molecular life sciences : CMLS · 2020Review
- The M1 muscarinic acetylcholine receptor in the crypt stem cell compartment mediates intestinal mucosal growth.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Prox1-positive cells monitor and sustain the murine intestinal epithelial cholinergic niche.Nature communications · 2020Article
- Mucins in Intestinal Mucosal Defense and Inflammation: Learning From Clinical and Experimental Studies.Frontiers in immunology · 2020Review
- The Role of the Gastrointestinal Mucus System in Intestinal Homeostasis: Implications for Neurological Disorders.Frontiers in cellular and infection microbiology · 2020Review
- Neurons and Glia in the Enteric Nervous System and Epithelial Barrier Function.Physiology (Bethesda, Md.) · 2018Review
- Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells.Cold Spring Harbor perspectives in medicine · 2017Review
- Dclk1-expressing tuft cells: critical modulators of the intestinal niche?American journal of physiology. Gastrointestinal and liver physiology · 2017Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe maintenance of the intestinal epithelium is of great importance for the survival of the organism. A possible nervous control of epithelial cell renewal was studied in rats and mice.
methodsMucosal afferent nerves were stimulated by exposing the intestinal mucosa to capsaicin (1.6 mM), which stimulates intestinal external axons. Epithelial cell renewal was investigated in the jejunum by measuring intestinal thymidine kinase (TK) activity, intestinal (3)H-thymidine incorporation into DNA, and the number of crypt cells labeled with BrdU. The influence of the external gut innervation was minimized by severing the periarterial nerves. PRINCIPAL
findingsLuminal capsaicin increased all the studied variables, an effect nervously mediated to judge from inhibitory effects on TK activity or (3)H-thymidine incorporation into DNA by exposing the mucosa to lidocaine (a local anesthetic) or by giving four different neurotransmitter receptor antagonists i.v. (muscarinic, nicotinic, neurokinin1 (NK1) or calcitonin gene related peptide (CGRP) receptors). After degeneration of the intestinal external nerves capsaicin did not increase TK activity, suggesting the involvement of an axon reflex. Intra-arterial infusion of Substance P (SP) or CGRP increased intestinal TK activity, a response abolished by muscarinic receptor blockade. Immunohistochemistry suggested presence of M3 and M5 muscarinic receptors on the intestinal stem/progenitor cells. We propose that the stem/progenitor cells are controlled by cholinergic nerves, which, in turn, are influenced by mucosal afferent neuron(s) releasing acetylcholine and/or SP and/or CGRP. In mice lacking the capsaicin receptor, thymidine incorporation into DNA and number of crypt cells labeled with BrdU was lower than in wild type animals suggesting that nerves are important also in the absence of luminal capsaicin, a conclusion also supported by the observation that atropine lowered thymidine incorporation into DNA by 60% in control rat segments.
conclusionEnteric nerves are of importance in maintaining the intestinal epithelial barrier.
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