ArticlePloS one2011
The PE-PPE domain in mycobacterium reveals a serine α/β hydrolase fold and function: an in-silico analysis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- ThemBio · 2026Article
- Sigma factor M induces an ESX-4-focused regulon inFrontiers in tuberculosis · 2026Article
- Pyrosequencing analysis of bacterial community changes in dental unit waterlines after chlorogenic acid treatment.Frontiers in cellular and infection microbiology · 2024Article
- Article
- PE_PGRS33, an Important Virulence Factor ofCells · 2021Review
- The final assembly of trehalose polyphleates takes place within the outer layer of the mycobacterial cell envelope.The Journal of biological chemistry · 2020Article
- Zinc binding proteome of a phytopathogenRoyal Society open science · 2019Article
- Microbial esterases and ester prodrugs: An unlikely marriage for combating antibiotic resistance.Drug development research · 2019Review
- Unsupervised statistical discovery of spaced motifs in prokaryotic genomes.BMC genomics · 2017Article
- Inactivation of MSMEG_0412 gene drastically affects surface related properties of Mycobacterium smegmatis.BMC microbiology · 2016Article
- Prediction of Certain Well-Characterized Domains of Known Functions within the PE and PPE Proteins of Mycobacteria.PloS one · 2016Article
- Mycobacterium tuberculosis Resists Stress by Regulating PE19 Expression.Infection and immunity · 2015Article
- Structure of EspB, a secreted substrate of the ESX-1 secretion system of Mycobacterium tuberculosis.Journal of structural biology · 2015Article
- Identification of novel Mycobacterium tuberculosis CD4 T-cell antigens via high throughput proteome screening.Tuberculosis (Edinburgh, Scotland) · 2015Article
- The rv1184c locus encodes Chp2, an acyltransferase in Mycobacterium tuberculosis polyacyltrehalose lipid biosynthesis.Journal of bacteriology · 2015Article
- Structure of the Mycobacterium tuberculosis type VII secretion system chaperone EspG5 in complex with PE25-PPE41 dimer.Molecular microbiology · 2014Article
- Article
- Putative roles of a proline-glutamic acid-rich protein (PE3) in intracellular survival and as a candidate for subunit vaccine against Mycobacterium tuberculosis.Medical microbiology and immunology · 2013Article
- The Nocardia cyriacigeorgica GUH-2 genome shows ongoing adaptation of an environmental Actinobacteria to a pathogen's lifestyle.BMC genomics · 2013Article
- The PE16 (Rv1430) of Mycobacterium tuberculosis is an esterase belonging to serine hydrolase superfamily of proteins.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The PE and PPE proteins first reported in the genome sequence of Mycobacterium tuberculosis strain H37Rv are now identified in all mycobacterial species. The PE-PPE domain (Pfam ID: PF08237) is a 225 amino acid residue conserved region located towards the C-terminus of some PE and PPE proteins and hypothetical proteins. Our in-silico sequence analysis revealed that this domain is present in all Mycobacteria, some Rhodococcus and Nocardia farcinica genomes. This domain comprises a pentapeptide sequence motif GxSxG/S at the N-terminus and conserved amino acid residues Ser, Asp and His that constitute a catalytic triad characteristic of lipase, esterase and cutinase activity. The fold prediction and comparative modeling of the 3-D structure of the PE-PPE domain revealed a "serine α/β hydrolase" structure with a central β-sheet flanked by α-helices on either side. The structure comprises a lid insertion with a closed structure conformation and has a solvent inaccessible active site. The oxyanion hole that stabilizes the negative charge on the tetrahedral intermediate has been identified. Our findings add to the growing list of serine hydrolases in mycobacterium, which are essential for the maintenance of their impermeable cell wall and virulence. These results provide the directions for the design of experiments to establish the function of PE and PPE proteins.
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