ArticlePloS one2011
Extracellular DNA is essential for maintaining Bordetella biofilm integrity on abiotic surfaces and in the upper respiratory tract of mice.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 115 citations in OpenAlex.
- Impact of Extracellular DNA on Architectural Parameters ofIndian journal of microbiology · 2023Article
- Secreted mammalian DNases protect against systemic bacterial infection by digesting biofilms.The Journal of experimental medicine · 2023Article
- The Role of Biofilms in the Pathogenesis of Animal Bacterial Infections.Microorganisms · 2023Review
- Architecture and matrix assembly determinants of Bordetella pertussis biofilms on primary human airway epithelium.PLoS pathogens · 2023Article
- Integrating proteomic data with metabolic modeling provides insight into key pathways ofFrontiers in microbiology · 2023Article
- Serum proteases prevent bacterial biofilm formation: role of kallikrein and plasmin.Virulence · 2021Article
- Pathogenicity and virulence ofVirulence · 2021Review
- AreViruses · 2021Article
- Microbial Biofilms in the Food Industry-A Comprehensive Review.International journal of environmental research and public health · 2021Review
- Antibiofilm Peptides: Relevant Preclinical Animal Infection Models and Translational Potential.ACS pharmacology & translational science · 2021Review
- Review
- The origin of extracellular DNA in bacterial biofilm infections in vivo.Pathogens and disease · 2020Article
- Structural mechanism for regulation of DNA binding of BpsR, a Bordetella regulator of biofilm formation, by 6-hydroxynicotinic acid.PloS one · 2019Article
- Structural Analysis of Bordetella pertussis Biofilms by Confocal Laser Scanning MicroscopyBio-protocol · 2018Article
- Extracellular DNA in natural environments: features, relevance and applications.Applied microbiology and biotechnology · 2018Review
- PgaB orthologues contain a glycoside hydrolase domain that cleaves deacetylated poly-β(1,6)-N-acetylglucosamine and can disrupt bacterial biofilms.PLoS pathogens · 2018Article
- Bordetella Pertussis virulence factors in the continuing evolution of whooping cough vaccines for improved performance.Medical microbiology and immunology · 2018Review
- Current therapies in treatment and prevention of fracture wound biofilms: why a multifaceted approach is essential for resolving persistent infections.Journal of bone and joint infection · 2018Review
- Effect of DNase Treatment on Adhesion and Early Biofilm Formation ofEuropean endodontic journal · 2018Article
- Hyperbiofilm Formation by Bordetella pertussis Strains Correlates with Enhanced Virulence Traits.Infection and immunity · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Bacteria form complex and highly elaborate surface adherent communities known as biofilms which are held together by a self-produced extracellular matrix. We have previously shown that by adopting a biofilm mode of existence in vivo, the gram negative bacterial pathogens Bordetella bronchiseptica and Bordetella pertussis are able to efficiently colonize and persist in the mammalian respiratory tract. In general, the bacterial biofilm matrix includes polysaccharides, proteins and extracellular DNA (eDNA). In this report, we investigated the function of DNA in Bordetella biofilm development. We show that DNA is a significant component of Bordetella biofilm matrix. Addition of DNase I at the initiation of biofilm growth inhibited biofilm formation. Treatment of pre-established mature biofilms formed under both static and flow conditions with DNase I led to a disruption of the biofilm biomass. We next investigated whether eDNA played a role in biofilms formed in the mouse respiratory tract. DNase I treatment of nasal biofilms caused considerable dissolution of the biofilm biomass. In conclusion, these results suggest that eDNA is a crucial structural matrix component of both in vitro and in vivo formed Bordetella biofilms. This is the first evidence for the ability of DNase I to disrupt bacterial biofilms formed on host organs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.