ArticlePlant physiology2011
Enzymatic activity of the soybean ecto-apyrase GS52 is essential for stimulation of nodulation.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Apyrase in horticultural crops: insights into growth, stress adaptation and quality regulation.Molecular horticulture · 2025Review
- Extracellular ATP: an emerging multifaceted regulator of plant fitness.Plant biotechnology journal · 2025Review
- Modified pea apyrase has altered nuclear functions and enhances the growth of yeast and Arabidopsis.Frontiers in plant science · 2025Article
- ATP homeostasis and signaling in plants.Plant communications · 2024Review
- Extracellular ATP targets Arabidopsis RIBONUCLEASE 1 to suppress mycotoxin stress-induced cell death.The New phytologist · 2022Article
- APYRASE1/2 mediate red light-induced de-etiolation growth in Arabidopsis seedlings.Plant physiology · 2022Article
- Progress in soybean functional genomics over the past decade.Plant biotechnology journal · 2022Review
- Recent Advances Clarifying the Structure and Function of Plant Apyrases (Nucleoside Triphosphate Diphosphohydrolases).International journal of molecular sciences · 2021Review
- ATP as Phosphorus and Nitrogen Source for Nutrient Uptake byFrontiers in plant science · 2019Article
- Populus euphratica APYRASE2 Enhances Cold Tolerance by Modulating Vesicular Trafficking and Extracellular ATP in Arabidopsis Plants.Plant physiology · 2015Article
- Effect of lipo-chitooligosaccharide on early growth of C4 grass seedlings.Journal of experimental botany · 2015Article
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- Chaperones are necessary for the expression of catalytically active potato apyrases in prokaryotic cells.Applied biochemistry and biotechnology · 2014Article
- Extracellular ATP acts as a damage-associated molecular pattern (DAMP) signal in plants.Frontiers in plant science · 2014Review
- Role of LysM receptors in chitin-triggered plant innate immunity.Plant signaling & behavior · 2013Review
- Rhizobial and mycorrhizal symbioses in Lotus japonicus require lectin nucleotide phosphohydrolase, which acts upstream of calcium signaling.Plant physiology · 2013Article
- 14-3-3 proteins SGF14c and SGF14l play critical roles during soybean nodulation.Plant physiology · 2012Article
- Purification and biochemical characterization of a novel ecto-apyrase, MP67, from Mimosa pudica.Plant physiology · 2011Article
- Enzymatic role for soybean ecto-apyrase in nodulation.Plant signaling & behavior · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Nitrogen is an essential nutrient for plant growth. In the Rhizobium-legume symbiosis, root nodules are the sites of bacterial nitrogen fixation, in which atmospheric nitrogen is converted into a form that plants can utilize. While recent studies suggested an important role for the soybean (Glycine max) ecto-apyrase GS52 in rhizobial root hair infection and root nodule formation, precisely how this protein impacts the nodulation process remains undetermined. In this study, the biochemical characteristics of the GS52 enzyme were investigated. Computer modeling of the GS52 apyrase structure identified key amino acid residues important for catalytic activity, which were subsequently mutagenized. Although the GS52 enzyme exhibited broad substrate specificity, its activity on pyrimidine nucleotides and diphosphate nucleotides was significantly higher than on ATP. This result was corroborated by structural modeling of GS52, which predicted a low specificity for the adenine base within the substrate-binding pocket of the enzyme. The wild-type enzyme and its inactive mutant forms were expressed in soybean roots in order to evaluate the importance of GS52 enzymatic activity for nodulation. The results indicated a clear correlation between GS52 enzymatic activity and nodule number. Altogether, our study indicates that the catalytic activity of the GS52 apyrase, likely acting on extracellular nucleotides, is critical for rhizobial infection and nodulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.