ArticleJournal of virology2011
Analysis of poliovirus protein 3A interactions with viral and cellular proteins in infected cells.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 50 citations in OpenAlex.
- Screening of Insertion Sites and Tags on EV-A71 VP1 Protein for Recombinant Virus Construction.Viruses · 2025Article
- Antiviral Development for the Polio Endgame: Current Progress and Future Directions.Pathogens (Basel, Switzerland) · 2024Review
- Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.The Journal of cell biology · 2022Article
- Manipulation of Host Cell Organelles by Intracellular Pathogens.International journal of molecular sciences · 2021Review
- Picornaviruses: A View from 3A.Viruses · 2021Review
- Calicivirus Non-structural Proteins: Potential Functions in Replication and Host Cell Manipulation.Frontiers in microbiology · 2021Review
- Enterovirus Infection Induces Massive Recruitment of All Isoforms of Small Cellular Arf GTPases to the Replication Organelles.Journal of virology · 2020Article
- Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality.bioRxiv : the preprint server for biology · 2020Article
- The Establishment of Infectious Clone and Single Round Infectious Particles for Coxsackievirus A10.Virologica Sinica · 2020Article
- A Redundant Mechanism of Recruitment Underlies the Remarkable Plasticity of the Requirement of Poliovirus Replication for the Cellular ArfGEF GBF1.Journal of virology · 2019Article
- Involvement of a Nonstructural Protein in Poliovirus Capsid Assembly.Journal of virology · 2019Article
- Construction of a recombinant rhinovirus accommodating fluorescent marker expression.Influenza and other respiratory viruses · 2018Article
- Phospholipid synthesis fueled by lipid droplets drives the structural development of poliovirus replication organelles.PLoS pathogens · 2018Article
- A Selective Bottleneck Shapes the Evolutionary Mutant Spectra of Enterovirus A71 during Viral Dissemination in Humans.Journal of virology · 2017Article
- Membrane alterations induced by nonstructural proteins of human norovirus.PLoS pathogens · 2017Article
- Involvement of a joker mutation in a polymerase-independent lethal mutagenesis escape mechanism.Virology · 2016Article
- Cell-specific establishment of poliovirus resistance to an inhibitor targeting a cellular protein.Journal of virology · 2015Article
- Picornavirus--host interactions to construct viral secretory membranes.Progress in molecular biology and translational science · 2015Review
- Lipids and RNA virus replication.Current opinion in virology · 2014Review
- Human rhinovirus 16 causes Golgi apparatus fragmentation without blocking protein secretion.Journal of virology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Poliovirus proteins 3A and 3AB are small, membrane-binding proteins that play multiple roles in viral RNA replication complex formation and function. In the infected cell, these proteins associate with other viral and cellular proteins as part of a supramolecular complex whose structure and composition are unknown. We isolated viable viruses with three different epitope tags (FLAG, hemagglutinin [HA], and c-myc) inserted into the N-terminal region of protein 3A. These viruses exhibited growth properties and characteristics very similar to those of the wild-type, untagged virus. Extracts prepared from the infected cells were subjected to immunoaffinity purification of the tagged proteins by adsorption to commercial antibody-linked beads and examined after elution for cellular and other viral proteins that remained bound to 3A sequences during purification. Viral proteins 2C, 2BC, 3D, and 3CD were detected in all three immunopurified 3A samples. Among the cellular proteins previously reported to interact with 3A either directly or indirectly, neither LIS1 nor phosphoinositol-4 kinase (PI4K) were detected in any of the purified tagged 3A samples. However, the guanine nucleotide exchange factor GBF1, which is a key regulator of membrane trafficking in the cellular protein secretory pathway and which has been shown previously to bind enteroviral protein 3A and to be required for viral RNA replication, was readily recovered along with immunoaffinity-purified 3A-FLAG. Surprisingly, we failed to cocapture GBF1 with 3A-HA or 3A-myc proteins. A model for variable binding of these 3A mutant proteins to GBF1 based on amino acid sequence motifs and the resulting practical and functional consequences thereof are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.