ArticleBiologicals : journal of the International Association of Biological Standardization2011
Development of a real-time reverse-transcription-PCR method for detection of RD114 virus in canine vaccines.
Article in Biologicals : journal of the International Association of Biological Standardization, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Article
- Genetic re-direction of canine primary T cells for clinical trial use in pet dogs with spontaneous cancer.STAR protocols · 2021Article
- Advances and insights in the diagnosis of viral infections.Journal of nanobiotechnology · 2021Review
- Current methods and prospects of coronavirus detection.Talanta · 2021Review
- Contamination of live attenuated vaccines with an infectious feline endogenous retrovirus (RD-114 virus).Archives of virology · 2014Review
- A new approach to establish a cell line with reduced risk of endogenous retroviruses.PloS one · 2013Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is known that certain feline cell lines, such as the Crandell-Rees feline kidney cell, produce an RD-114-like virus. As a feline endogenous retrovirus, RD114 virus, exists in the genome of all cats, it can be assumed that contamination with the virus in feline and canine live vaccines manufactured by culturing cells of feline origin occurs. To detect an infectious RD114 virus in vitro, a LacZ marker rescue assay has recently been established. In feline and canine live vaccines approved in Japan, feline cell lines are widely used to produce vaccines, especially those containing canine parvovirus components. The LacZ marker rescue assay detects infectious viral particles, but the real-time reverse-transcription-PCR detects both infectious and defective viruses. The canine live vaccines manufactured in cells of feline origin showed positive results for the env gene by the real-time reverse-transcription-PCR, including all of the 8 vaccines produced in feline cell lines that were negative in the LacZ marker rescue assay. In conclusion, the present investigation suggests that the newly developed method has the advantages of shorter time requirements and can be applied as a valuable screening method to detect RD114 viral RNA in vaccines.
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Registered trials
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