ArticleActa tropica2011
Evaluation of a PCR-RFLP-ITS2 assay for discrimination of Anopheles species in northern and western Colombia.
Article in Acta tropica, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Discrimination of Hard Ticks by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).International journal of molecular sciences · 2025Article
- An updated checklist ofZooKeys · 2025Article
- Malaria Vectors andTropical medicine and infectious disease · 2024Article
- Spatial and Temporal Diversity Variation in the Anopheles Communities in Malaria-Endemic Regions of Colombia.The American journal of tropical medicine and hygiene · 2023Article
- PCR-RFLP assays for the identification of Anopheles (Diptera: Culicidae) species circulating in Honduras.Malaria journal · 2023Article
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- Factors shaping the gut bacterial community assembly in two main Colombian malaria vectors.Microbiome · 2018Article
- Article
- Potential distribution of mosquito vector species in a primary malaria endemic region of Colombia.PloS one · 2017Article
- Epidemic and Non-Epidemic Hot Spots of Malaria Transmission Occur in Indigenous Comarcas of Panama.PLoS neglected tropical diseases · 2016Article
- Epidemiological and entomological studies of a malaria outbreak among French armed forces deployed at illegal gold mining sites reveal new aspects of the disease's transmission in French Guiana.Malaria journal · 2016Article
- An overview of malaria transmission from the perspective of Amazon Anopheles vectors.Memorias do Instituto Oswaldo Cruz · 2015Review
- Molecular taxonomy provides new insights into anopheles species of the neotropical arribalzagia series.PloS one · 2015Article
- Anthropophilic Anopheles species composition and malaria in Tierradentro, Córdoba, Colombia.Memorias do Instituto Oswaldo Cruz · 2014Article
- Malaria vectors in ecologically heterogeneous localities of the Colombian Pacific region.PloS one · 2014Article
- Article
- Abundance, behavior and entomological inoculation rates of anthropophilic anophelines from a primary Colombian malaria endemic area.Parasites & vectors · 2013Article
- Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns.Parasites & vectors · 2013Article
- Molecular taxonomy of Anopheles (Nyssorhynchus) benarrochi (Diptera: Culicidae) and malaria epidemiology in southern Amazonian Peru.The American journal of tropical medicine and hygiene · 2013Article
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6 authors.
Funding
Abstract
Anopheles mosquitoes are routinely identified using morphological characters of the female that often lead to misidentification due to interspecies similarity and intraspecies variability. The aim of this work was to evaluate the applicability of a previously developed PCR-RFLP-ITS2 assay for accurate discrimination of anophelines in twelve localities spanning three Colombian malaria epidemiological regions: Atlantic Coast, Pacific Coast, and Uraba-Bajo Cauca-Alto Sinu region. The evaluation of the stability of the PCR-RFLP patterns is required since variability of the ITS2 has been documented and may produce discrepancies in the patterns previously reported. The assay was used to evaluate species assignation of 939 mosquitoes identified by morphology. Strong agreement between the morphological and molecular identification was found for species Anopheles albimanus, Anopheles aquasalis, Anopheles darlingi and Anopheles triannulatus s.l. (p≥0.05, kappa=1). However, disagreement was found for species Anopheles nuneztovari s.l., Anopheles neomaculipalpus, Anopheles apicimacula and Anopheles punctimacula (p≤0.05; kappa ranging from 0.33 to 0.80). The ITS2-PCR-RFLP assay proved valuable for discriminating anopheline species of northern and western Colombia, especially those with overlapping morphology in the Oswaldoi Group.
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