ArticleVeterinary research2011
In vitro invasion efficiency and intracellular proliferation rate comprise virulence-related phenotypic traits of Neospora caninum.
Article in Veterinary research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Serological detection ofOpen veterinary journal · 2025Article
- Loss of NcBPK1 impairs bradyzoite differentiation and enhances virulence in Neospora caninum.Parasites & vectors · 2025Article
- TaqMan-quantitative PCR assays applied inFrontiers in cellular and infection microbiology · 2024Article
- Effects of Ovine Monocyte-Derived Macrophage Infection by Recently IsolatedAnimals : an open access journal from MDPI · 2022Article
- NcPathogens (Basel, Switzerland) · 2022Article
- Parasitemia and Associated Immune Response in Pregnant and Non-Pregnant Beef Cows Naturally Infected WithFrontiers in veterinary science · 2022Article
- Unifying Virulence Evaluation inFrontiers in cellular and infection microbiology · 2022Review
- Dynamics ofPathogens (Basel, Switzerland) · 2021Article
- In vivo and in vitro models show unexpected degrees of virulence among Toxoplasma gondii type II and III isolates from sheep.Veterinary research · 2021Article
- Spatial distance between sites of sampling associated with genetic variation among Neospora caninum in aborted bovine foetuses from northern Italy.Parasites & vectors · 2021Article
- Maternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates ofFrontiers in cellular and infection microbiology · 2021Article
- Proteomic Characterization of Host-Pathogen Interactions during Bovine Trophoblast Cell Line Infection byPathogens (Basel, Switzerland) · 2020Article
- Neospora caninum infection induces an isolate virulence-dependent pro-inflammatory gene expression profile in bovine monocyte-derived macrophages.Parasites & vectors · 2020Article
- Crosstalk between Neospora caninum and the bovine host at the maternal-foetal interface determines the outcome of infection.Veterinary research · 2020Article
- Modeling the Ruminant Placenta-Pathogen Interactions in Apicomplexan Parasites: Current and Future Perspectives.Frontiers in veterinary science · 2020Review
- Lytic cycle of Besnoitia besnoiti tachyzoites displays similar features in primary bovine endothelial cells and fibroblasts.Parasites & vectors · 2019Article
- Immune response to Neospora caninum live tachyzoites in prepubertal female calves.Parasitology research · 2019Article
- Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates.Veterinary research · 2019Article
- Immune response profile of caruncular and trophoblast cell lines infected by high- (Nc-Spain7) and low-virulence (Nc-Spain1H) isolates of Neospora caninum.Parasites & vectors · 2019Article
- Gene Expression Profiling ofFrontiers in cellular and infection microbiology · 2019Article
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7 authors.
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Abstract
In this study, we examined the in vitro invasion and proliferation capacities of the Nc-Liv and ten Spanish Neospora caninum isolates (Nc-Spain 1 H - Nc-Spain 10). The invasion rate was determined as the number of tachyzoites that completed their internalisation into MARC-145 cells at 2, 4, and 6 h post-inoculation (pi). The proliferation rate was evaluated by determining the doubling time during the exponential proliferation period. Significant differences in the invasion rates of these isolates were detected at 2 and 4 h pi (P < 0.0001, Kruskal-Wallis test). At 4 h pi, the Nc-Spain 4 H and Nc-Liv isolates displayed the highest, while the Nc-Spain 3 H and Nc-Spain 1 H isolates had the lowest invasion rates (by Dunn's test). Variations in the proliferation kinetics of these isolates were also observed. Between different isolates, the lag phase, which occurs before the exponential growth phase, ranged from 8 to 44 h, and the doubling time ranged from 9.8 to 14.1 h (P = 0.0016, ANOVA test). Tachyzoite yield, which combines invasion and proliferation data, was also assessed and confirmed marked differences between the highly and less prolific isolates. Interestingly, a direct correlation between the invasion rates and tachyzoite yields, and the severity of the disease that was exhibited by infected pregnant mice in previous works could be established for the isolates in this study (Spearman's coefficient > 0.62, P < 0.05). The results of this study may help us to explain the differences in the pathogenicity that are displayed by different isolates.
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