ArticleAnalytical chemistry2011
Generating nonlinear concentration gradients in microfluidic devices for cell studies.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Spatially resolved analysis of microenvironmental gradient impact on cancer cell phenotypes.Science advances · 2024Article
- High-throughput co-culture system for analysis of spatiotemporal cell-cell signaling.Biosensors & bioelectronics · 2023Article
- Review
- Patterning Neuroepithelial Cell SheetACS biomaterials science & engineering · 2021Article
- Gradient Hydrogels.Advances in biochemical engineering/biotechnology · 2021Article
- Functionally graded biomaterials for use as model systems and replacement tissues.Advanced functional materials · 2020Article
- Gradient Material Strategies for Hydrogel Optimization in Tissue Engineering Applications.High-throughput · 2018Review
- Arbitrarily Accessible 3D Microfluidic Device for Combinatorial High-Throughput Drug Screening.Sensors (Basel, Switzerland) · 2016Article
- Combinatorial Method/High Throughput Strategies for Hydrogel Optimization in Tissue Engineering Applications.Gels (Basel, Switzerland) · 2016Review
- Flow distribution in parallel microfluidic networks and its effect on concentration gradient.Biomicrofluidics · 2015Article
- A Current View of Functional Biomaterials for Wound Care, Molecular and Cellular Therapies.BioMed research international · 2015Review
- Shape-controlled synthesis of hybrid nanomaterials via three-dimensional hydrodynamic focusing.ACS nano · 2014Article
- A multistage volumetric bar chart chip for visualized quantification of DNA.Journal of the American Chemical Society · 2013Article
- Enabling systems biology approaches through microfabricated systems.Analytical chemistry · 2013Article
- Microengineered tumor models: insights & opportunities from a physical sciences-oncology perspective.Biomedical microdevices · 2013Review
- Tunable, pulsatile chemical gradient generation via acoustically driven oscillating bubbles.Lab on a chip · 2013Article
- A mini-microscope for in situ monitoring of cells.Lab on a chip · 2012Article
- Material strategies for creating artificial cell-instructive niches.Current opinion in biotechnology · 2012Review
- Polyester μ-assay chip for stem cell studies.Biomicrofluidics · 2012Article
- From 3D cell culture to organs-on-chips.Trends in cell biology · 2011Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
We describe a microfluidic device for generating nonlinear (exponential and sigmoidal) concentration gradients, coupled with a microwell array for cell storage and analysis. The device has two inputs for coflowing multiple aqueous solutions, a main coflow channel and an asymmetrical grid of fluidic channels that allows the two solutions to combine at intersection points without fully mixing. Due to this asymmetry and diffusion of the two species in the coflow channel, varying amounts of the two solutions enter each fluidic path. This induces exponential and sigmoidal concentration gradients at low and high flow rates, respectively, making the microfluidic device versatile. A key feature of this design is that it is space-saving, as it does not require multiplexing or a separate array of mixing channels. Furthermore, the gradient structure can be utilized in concert with cell experiments, to expose cells captured in microwells to various concentrations of soluble factors. We demonstrate the utility of this design to assess the viability of fibroblast cells in response to a range of hydrogen peroxide (H(2)O(2)) concentrations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.