ArticleMycorrhiza2011
Optimized assay and storage conditions for enzyme activity profiling of ectomycorrhizae.
Article in Mycorrhiza, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 72 citations in OpenAlex.
- Exploration capacity versus specific enzymatic activity of ectomycorrhizas in response to primary productivity and soil phosphorus availability in Bornean tropical rainforests.Scientific reports · 2024Article
- Soil Storage Conditions Alter the Effects of Tire Wear Particles on Microbial Activities in Laboratory Tests.Environmental science & technology letters · 2022Article
- Micro-scale Experimental System Coupled with Fluorescence-based Estimation of Fungal Biomass to Study Utilisation of Plant Substrates.Microbial ecology · 2022Article
- Scions impact biomass allocation and root enzymatic activity of rootstocks in grafted melon and watermelon plants.Frontiers in plant science · 2022Article
- Factors influencing successful establishment of exoticFrontiers in microbiology · 2022Article
- Ectomycorrhizal Networks in the Anthropocene: From Natural Ecosystems to Urban Planning.Frontiers in plant science · 2022Review
- Phosphorus deficiencies invoke optimal allocation of exoenzymes by ectomycorrhizas.The ISME journal · 2021Article
- Article
- The Free-Living Stage Growth Conditions of the Endophytic FungusFrontiers in microbiology · 2020Article
- Plant roots increase both decomposition and stable organic matter formation in boreal forest soil.Nature communications · 2019Article
- Polyporales Brown Rot Species Fomitopsis pinicola: Enzyme Activity Profiles, Oxalic Acid Production, and FeApplied and environmental microbiology · 2018Article
- Article
- Environmental and microbial factors influencing methane and nitrous oxide fluxes in Mediterranean cork oak woodlands: trees make a difference.Frontiers in microbiology · 2015Article
- Loss of diversity in wood-inhabiting fungal communities affects decomposition activity in Norway spruce wood.Frontiers in microbiology · 2014Article
- The ectomycorrhizal fungus Tricholoma matsutake is a facultative saprotroph in vitro.Mycorrhiza · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
22 authors at 11 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of a joint effort by different research teams was to provide an improved procedure for enzyme activity profiling of field-sampled ectomycorrhizae, including recommendations on the best conditions and maximum duration for storage of ectomycorrhizal samples. A more simplified and efficient protocol compared to formerly published procedures was achieved by using manufactured 96-filter plates in combination with a vacuum manifold and by optimizing incubation times. Major improvements were achieved by performing the series of eight enzyme assays with a single series of root samples instead of two series, reducing the time needed for sample preparation, minimizing error-prone steps such as pipetting and morphotyping, and facilitating subsequent DNA analyses due to the reduced sequencing effort. The best preservation of samples proved to be storage in soil at 4-6 °C in the form of undisturbed soil cores containing roots. Enzyme activities were maintained for up to 4 weeks under these conditions. Short-term storage of washed roots and ectomycorrhizal tips overnight in water did not cause substantial changes in enzyme activity profiles. No optimal means for longer-term storage by freezing at -20 °C or storage in 100% ethanol were recommended.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.