ArticleThe EMBO journal2011
The RSC chromatin remodelling enzyme has a unique role in directing the accurate positioning of nucleosomes.
Article in The EMBO journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 55 citations in OpenAlex.
- Poly(dA:dT) Tracts Differentially Modulate Nucleosome Remodeling Activity of RSC and ISW1a Complexes, Exerting Tract Orientation-Dependent and -Independent Effects.International journal of molecular sciences · 2023Article
- Nucleosome Remodeling at the YeastInternational journal of molecular sciences · 2023Article
- The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.Nucleic acids research · 2022Article
- Superstructure Detection in Nucleosome Distribution Shows Common Pattern within a Chromosome and within the Genome.Life (Basel, Switzerland) · 2022Article
- The Active Mechanism of Nucleosome Depletion by Poly(dA:dT) Tracts In Vivo.International journal of molecular sciences · 2021Review
- Ruler elements in chromatin remodelers set nucleosome array spacing and phasing.Nature communications · 2021Article
- Interaction of RSC Chromatin Remodeling Complex with Nucleosomes Is Modulated by H3 K14 Acetylation and H2B SUMOylation In Vivo.iScience · 2020Article
- Absolute nucleosome occupancy map for theGenome research · 2019Article
- Genome-wide reconstitution of chromatin transactions reveals that RSC preferentially disrupts H2AZ-containing nucleosomes.Genome research · 2019Article
- Sth1, the Key Subunit of the RSC Chromatin Remodeling Complex, Is Essential in Maintaining Chromosomal Integrity and Mediating High Fidelity Chromosome Segregation in the Human Fungal PathogenFrontiers in microbiology · 2019Article
- In silico evidence for sequence-dependent nucleosome sliding.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A conserved role of the RSC chromatin remodeler in the establishment of nucleosome-depleted regions.Current genetics · 2017Review
- Hsp90 and p23 Molecular Chaperones Control Chromatin Architecture by Maintaining the Functional Pool of the RSC Chromatin Remodeler.Molecular cell · 2016Article
- Article
- Functional compensation among HMGN variants modulates the DNase I hypersensitive sites at enhancers.Genome research · 2015Article
- Nucleosome positioning in yeasts: methods, maps, and mechanisms.Chromosoma · 2015Review
- H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes.Nucleic acids research · 2015Article
- Initiation of meiotic homologous recombination: flexibility, impact of histone modifications, and chromatin remodeling.Cold Spring Harbor perspectives in biology · 2015Review
- Nucleosome spacing generated by ISWI and CHD1 remodelers is constant regardless of nucleosome density.Molecular and cellular biology · 2015Article
- Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Nucleosomes impede access to DNA. Therefore, nucleosome positioning is fundamental to genome regulation. Nevertheless, the molecular nucleosome positioning mechanisms are poorly understood. This is partly because in vitro reconstitution of in vivo-like nucleosome positions from purified components is mostly lacking, barring biochemical studies. Using a yeast extract in vitro reconstitution system that generates in vivo-like nucleosome patterns at S. cerevisiae loci, we find that the RSC chromatin remodelling enzyme is necessary for nucleosome positioning. This was previously suggested by genome-wide in vivo studies and is confirmed here in vivo for individual loci. Beyond the limitations of conditional mutants, we show biochemically that RSC functions directly, can be sufficient, but mostly relies on other factors to properly position nucleosomes. Strikingly, RSC could not be replaced by either the closely related SWI/SNF or the Isw2 remodelling enzyme. Thus, we pinpoint that nucleosome positioning specifically depends on the unique properties of the RSC complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.