ArticleAntimicrobial agents and chemotherapy2011
Comparative genome sequencing of an isogenic pair of USA800 clinical methicillin-resistant Staphylococcus aureus isolates obtained before and after daptomycin treatment failure.
Article in Antimicrobial agents and chemotherapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 67 citations in OpenAlex.
- Expression ofmBio · 2025Article
- Characterisation ofJAC-antimicrobial resistance · 2025Article
- Emergence of transferable daptomycin resistance in Gram-positive bacteria.npj antimicrobials and resistance · 2025Article
- In Vivo-Acquired Resistance to Daptomycin during Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- Cervimycin-Resistant Staphylococcus aureus Strains Display Vancomycin-Intermediate Resistant Phenotypes.Microbiology spectrum · 2022Article
- Comparative Genomic Reveals Clonal Heterogeneity in PersistentFrontiers in cellular and infection microbiology · 2022Article
- Article
- MPC-Based Prediction of Anti-Mutant Effectiveness of Antibiotic Combinations: In Vitro Model Study with Daptomycin and Gentamicin againstAntibiotics (Basel, Switzerland) · 2021Article
- Genomic Insights Into Last-Line Antimicrobial Resistance in Multidrug-ResistantFrontiers in microbiology · 2021Review
- Article
- Metabolic changes associated with adaptive resistance to daptomycin in Streptococcus mitis-oralis.BMC microbiology · 2020Article
- Temperate Phages ofMicrobiology spectrum · 2019Review
- Metabolic interventions for the prevention and treatment of daptomycin non-susceptibility in Staphylococcus aureus.The Journal of antimicrobial chemotherapy · 2019Article
- Insights Into the Evolution ofFrontiers in microbiology · 2019Article
- Characterization of genetic changes associated with daptomycin nonsusceptibility in Staphylococcus aureus.PloS one · 2018Article
- Effect of a Point Mutation inFrontiers in microbiology · 2018Article
- Metabolic Perturbations in a Bacillus subtilis clpP Mutant during Glucose Starvation.Metabolites · 2017Article
- Staphylococcus aureus metabolic adaptations during the transition from a daptomycin susceptibility phenotype to a daptomycin nonsusceptibility phenotype.Antimicrobial agents and chemotherapy · 2015Article
- [Resistance to "last resort" antibiotics in Gram-positive cocci: The post-vancomycin era].Biomedica : revista del Instituto Nacional de Salud · 2014Review
- Classification of epidemic community-acquired methicillin-resistant Staphylococcus aureus by anatomical site of isolation.BioMed research international · 2014Article
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12 authors at 4 institutions in 1 country.
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Abstract
We describe here a clinical daptomycin treatment failure in a patient with recurrent methicillin-resistant Staphylococcus aureus (MRSA) bacteremia in whom daptomycin was administered after a failed empirical treatment course with vancomycin and piperacillin-tazobactam. We had the opportunity to compare the genome sequences of an isogenic pair of daptomycin-susceptible and -resistant MRSA isolates obtained before and after initiation of daptomycin therapy, respectively. The genotype of both isolates was USA800, ST5, SCCmec type IV, agr type II. There was no increase in cell wall thickness in the daptomycin-resistant strain despite having decreased susceptibility to both vancomycin and daptomycin. By comparing the genome sequences by pyrosequencing, we identified a polymorphism (S337L) in the tenth transmembrane segment of the multiple peptide resistance factor, MprF, encoding lysyl phosphatidylglycerol transferase. This enzyme has been shown previously to promote repulsion of daptomycin at the cell surface by addition of positively charged lysine to phosphatidylglycerol. Also, the hlb open reading frame (ORF) encoding the β-toxin was interrupted by a prophage in the daptomycin-susceptible strain; this phage was missing in the daptomycin-resistant isolate and the hlb ORF was restored. Loss of the phage in the resistant isolate also resulted in loss of the virulence factor genes clpP, scn, and sak. This is the first study to use pyrosequencing to compare the genomes of a daptomycin-susceptible/resistant MRSA isolate pair obtained during failed daptomycin therapy in humans.
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