ArticleIEEE transactions on bio-medical engineering2011
Development and initial results of a low cost, disposable, point-of-care testing device for pathogen detection.
Article in IEEE transactions on bio-medical engineering, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Rapid detection of Galba truncatula in water sources on pasture-land using loop-mediated isothermal amplification for control of trematode infections.Parasites & vectors · 2020Article
- Article
- Laboratory-Based and Point-of-Care Testing for MSSA/MRSA Detection in the Age of Whole Genome Sequencing.Frontiers in microbiology · 2018Review
- Evaluation of Two Lyophilized Molecular Assays to Rapidly Detect Foot-and-Mouth Disease Virus Directly from Clinical Samples in Field Settings.Transboundary and emerging diseases · 2017Article
- Detection of pathogen-specific antibodies by loop-mediated isothermal amplification.Clinical and vaccine immunology : CVI · 2015Article
- Article
- Nucleic-acid testing, new platforms and nanotechnology for point-of-decision diagnosis of animal pathogens.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Molecular-based isothermal tests for field diagnosis of malaria and their potential contribution to malaria elimination.The Journal of antimicrobial chemotherapy · 2015Review
- A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods.Biomolecular detection and quantification · 2014Article
- Loop-mediated isothermal amplification for Rickettsia typhi (the causal agent of murine typhus): problems with diagnosis at the limit of detection.Journal of clinical microbiology · 2014Article
- Article
- Advances in tuberculosis diagnostics: the Xpert MTB/RIF assay and future prospects for a point-of-care test.The Lancet. Infectious diseases · 2013Review
- Miniaturized technology for protein and nucleic acid point-of-care testing.Translational research : the journal of laboratory and clinical medicine · 2012Review
- Instrument-free nucleic acid amplification assays for global health settings.Proceedings of SPIE--the International Society for Optical Engineering · 2011Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Development of small footprint, disposable, fast, and inexpensive devices for pathogen detection in the field and clinic would benefit human and veterinary medicine by allowing evidence-based responses to future out breaks. We designed and tested an integrated nucleic acid extraction and amplification device employing a loop-mediated isothermal amplification (LAMP) or reverse transcriptase-LAMP assay. Our system provides a screening tool with polymerase-chain-reaction-level sensitivity and specificity for outbreak detection, response, and recovery. Time to result is ∼90 min. The device utilizes a swab that collects sample and then transfers it to a disc of cellulose-based nucleic acid binding paper. The disc is positioned within a disposable containment tube with a manual loading port. In order to test for the presence of target pathogens, LAMP reagents are loaded through the tube's port into contact with the sample containing cellulose disc. The reagents then are isothermally heated to 63°C for ∼1 h to achieve sequence-specific target nucleic acid amplification. Due to the presence of a colorimetric dye, amplification induces visible color change in the reagents from purple to blue. As initial demonstrations, we detected methicillin resistant Staphylococcus aureus genomic DNA, as well as recombinant and live foot-and-mouth disease virus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.