ArticleBiosensors & bioelectronics2011
Label-free multiplexed virus detection using spectral reflectance imaging.
Article in Biosensors & bioelectronics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Optical Biosensors for the Diagnosis of COVID-19 and Other Viruses-A Review.Diagnostics (Basel, Switzerland) · 2023Review
- Solid-Phase Optical Sensing Techniques for Sensitive Virus Detection.Sensors (Basel, Switzerland) · 2023Review
- Challenges and perspectives of multi-virus biosensing techniques: A review.Analytica chimica acta · 2023Review
- Highly-Sensitive, Label-Free Detection of Microorganisms and Viruses via Interferometric Reflectance Imaging Sensor.Micromachines · 2023Review
- Current Advances in Technologies for Single Extracellular Vesicle Analysis and Its Clinical Applications in Cancer Diagnosis.Biosensors · 2023Review
- Recent Developments in the Field of Optical Immunosensors Focusing on a Label-Free, White Light Reflectance Spectroscopy-Based Immunosensing Platform.Sensors (Basel, Switzerland) · 2022Review
- Point-of-Care Diagnostics for Farm Animal Diseases: From Biosensors to Integrated Lab-on-Chip Devices.Biosensors · 2022Review
- Utilising extracellular vesicles for early cancer diagnostics: benefits, challenges and recommendations for the future.British journal of cancer · 2022Review
- The Effects of Three-Dimensional Ligand Immobilization on Kinetic Measurements in Biosensors.Polymers · 2022Article
- Multiplexed Affinity Measurements of Extracellular Vesicles Binding Kinetics.Sensors (Basel, Switzerland) · 2021Article
- Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single-particle analysis platforms.Journal of extracellular vesicles · 2021Article
- The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview.Polymers · 2021Review
- Engineering photonics solutions for COVID-19.APL photonics · 2020Review
- Full-field interferometry for counting and differentiating aquatic biotic nanoparticles: from laboratory toBiomedical optics express · 2016Article
- Interferometric Reflectance Imaging Sensor (IRIS)--A Platform Technology for Multiplexed Diagnostics and Digital Detection.Sensors (Basel, Switzerland) · 2015Review
- Advances in plasmonic technologies for point of care applications.Chemical reviews · 2014Review
- Article
- An interferometric reflectance imaging sensor for point of care viral diagnostics.IEEE transactions on bio-medical engineering · 2013Article
- Multiplexed method to calibrate and quantitate fluorescence signal for allergen-specific IgE.Analytical chemistry · 2011Article
- Biomolecular detection employing the Interferometric Reflectance Imaging Sensor (IRIS).Journal of visualized experiments : JoVE · 2011Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
We demonstrate detection of whole viruses and viral proteins with a new label-free platform based on spectral reflectance imaging. The Interferometric Reflectance Imaging Sensor (IRIS) has been shown to be capable of sensitive protein and DNA detection in a real time and high-throughput format. Vesicular stomatitis virus (VSV) was used as the target for detection as it is well-characterized for protein composition and can be modified to express viral coat proteins from other dangerous, highly pathogenic agents for surrogate detection while remaining a biosafety level 2 agent. We demonstrate specific detection of intact VSV virions achieved with surface-immobilized antibodies acting as capture probes which is confirmed using fluorescence imaging. The limit of detection is confirmed down to 3.5 × 10(5)plaque-forming units/mL (PFUs/mL). To increase specificity in a clinical scenario, both the external glycoprotein and internal viral proteins were simultaneously detected with the same antibody arrays with detergent-disrupted purified VSV and infected cell lysate solutions. Our results show sensitive and specific virus detection with a simple surface chemistry and minimal sample preparation on a quantitative label-free interferometric platform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.