ArticleAnalytical biochemistry2011
Linear nicking endonuclease-mediated strand-displacement DNA amplification.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Aptamers as target-specific recognition elements in drug delivery.Advanced drug delivery reviews · 2025Review
- CLAE: A High-Fidelity Nanopore Sequencing Strategy for Read-Level Viral Variant Detection and Environmental RNA Virus Discovery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- AMPLON: Amplifying DNA with Multiarm Priming and Looping Optimization of Nucleic Acid.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Coupling Exponential to Linear Amplification for Endpoint Quantitative Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- PhotocleavableChemical reviews · 2023Review
- Toward a next-generation diagnostic tool: A review on emerging isothermal nucleic acid amplification techniques for the detection of SARS-CoV-2 and other infectious viruses.Analytica chimica acta · 2022Review
- Types and Applications of Nicking Enzyme-Combined Isothermal Amplification.International journal of molecular sciences · 2022Review
- Article
- A Sensitive Thrombin Aptasensor Based on Target Circulation Strategy.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Graphene oxide assisted light-up aptamer selection against Thioflavin T for label-free detection of microRNA.Scientific reports · 2021Article
- Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage.Computational and structural biotechnology journal · 2021Review
- Synthesis of DNA Origami Scaffolds: Current and Emerging Strategies.Molecules (Basel, Switzerland) · 2020Review
- Transcriptomics in Alzheimer's Disease: Aspects and Challenges.International journal of molecular sciences · 2020Review
- Review
- A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment.Nucleic acids research · 2019Article
- A CRISPR-Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection.Nature communications · 2018Article
- Dna2 processes behind the fork long ssDNA flaps generated by Pif1 and replication-dependent strand displacement.Nature communications · 2018Article
- Isothermal amplification of long DNA segments by quadruplex priming amplification.Analytical methods : advancing methods and applications · 2018Article
- Scalable amplification of strand subsets from chip-synthesized oligonucleotide libraries.Nature communications · 2015Article
- A Major Locus Controls a Genital Shape Difference Involved in Reproductive Isolation Between Drosophila yakuba and Drosophila santomea.G3 (Bethesda, Md.) · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We describe a method for linear isothermal DNA amplification using nicking endonuclease-mediated strand displacement by a DNA polymerase. The nicking of one strand of a DNA target by the endonuclease produces a primer for the polymerase to initiate synthesis. As the polymerization proceeds, the downstream strand is displaced into a single-stranded form while the nicking site is also regenerated. The combined continuous repetitive action of nicking by the endonuclease and strand-displacement synthesis by the polymerase results in linear amplification of one strand of the DNA molecule. We demonstrate that DNA templates up to 5000 nucleotides can be linearly amplified using a nicking endonuclease with 7-bp recognition sequence and Sequenase version 2.0 in the presence of single-stranded DNA binding proteins. We also show that a mixture of three templates of 500, 1000, and 5000 nucleotides in length is linearly amplified with the original molar ratios of the templates preserved. Moreover, we demonstrate that a complex library of hydrodynamically sheared genomic DNA from bacteriophage lambda can be amplified linearly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.