ArticleTransgenic research2011
Anthocyanin production as a potential visual selection marker during plant transformation.
Article in Transgenic research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 78 citations in OpenAlex.
- Article
- A robust in-vitro and ex-vitro Agrobacterium rhizogenes-mediated hairy root transformation system in mungbean for efficient visual screening of transformants using the RUBY reporter.BMC plant biology · 2025Article
- Overview on Current Selectable Marker Systems and Novel Marker Free Approaches in Fruit Tree Genetic Engineering.International journal of molecular sciences · 2024Review
- An Anthocyanin-Based Visual Reporter System for Genetic Transformation and Genome Editing in Cassava.International journal of molecular sciences · 2024Article
- An efficient visual screening of gene knockout mutants in the insect pathogenic fungus Beauveria bassiana.Microbial biotechnology · 2024Article
- Generation of parthenocarpic tomato plants in multiple elite cultivars using the CRISPR/Cas9 system.Molecular breeding : new strategies in plant improvement · 2024Article
- Influence of Anthocyanin Expression on the Performance of Photosynthesis in Sweet Orange,Plants (Basel, Switzerland) · 2023Article
- Development of an inducible excision system of a visual markerPlant biotechnology (Tokyo, Japan) · 2023Article
- Cisgenics and intragenics: boon or bane for crop improvement.Frontiers in plant science · 2023Review
- Optimization ofFrontiers in plant science · 2023Article
- An Erwinia amylovora inducible promoter for improvement of apple fire blight resistance.Plant cell reports · 2022Article
- Chili PepperPlants (Basel, Switzerland) · 2022Article
- Expression of apple MdMYB10 transcription factor in sugar beet with a screenable marker role and antimicrobial activity.3 Biotech · 2022Article
- Article
- The Intragenesis and Synthetic Biology Approach towards Accelerating Genetic Gains on Strawberry: Development of New Tools to Improve Fruit Quality and Resistance to Pathogens.Plants (Basel, Switzerland) · 2021Article
- Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation.Plants (Basel, Switzerland) · 2021Review
- MYB-Mediated Regulation of Anthocyanin Biosynthesis.International journal of molecular sciences · 2021Review
- Article
- Article
- Genome Editing in Agriculture: Technical and Practical Considerations.International journal of molecular sciences · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A mutant allele of the transcription factor gene MYB10 from apple induces anthocyanin production throughout the plant. This gene, including its upstream promoter, gene coding region and terminator sequence, was introduced into apple, strawberry and potato plants to determine whether it could be used as a visible selectable marker for plant transformation as an alternative to chemically selectable markers, such as kanamycin resistance. After transformation, red coloured calli, red shoots and red well-growing plants were scored. Red and green shoots were harvested from apple explants and examined for the presence of the MYB10 gene by PCR analysis. Red shoots of apple explants always contained the MYB10 gene but not all MYB10 containing shoots were red. Strawberry plants transformed with the MYB10 gene showed anthocyanin accumulation in leaves and roots. No visible accumulation of anthocyanin could be observed in potato plants grown in vitro, even the ones carrying the MYB10 gene. However, acid methanol extracts of potato shoots or roots carrying the MYB10 gene contained up to four times higher anthocyanin content than control plants. Therefore anthocyanin production as result of the apple MYB10 gene can be used as a selectable marker for apple, strawberry and potato transformation, replacing kanamycin resistance.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.