ArticlePflugers Archiv : European journal of physiology2011
Bimodal effect of alkalization on the polycystin transient receptor potential channel, PKD2L1.
Article in Pflugers Archiv : European journal of physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Physiological functions and pharmacological targeting of transient receptor potential channels.Pharmacological reviews · 2025Review
- Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine.Scientific reports · 2023Article
- Polycystin Channel Complexes.Annual review of physiology · 2023Review
- Adaptive selection drives TRPP3 loss-of-function in an Ethiopian population.Scientific reports · 2020Article
- The asparagine 533 residue in the outer pore loop region of the mouse PKD2L1 channel is essential for its voltage-dependent inactivation.FEBS open bio · 2017Article
- Integrative Approach with Electrophysiological and Theoretical Methods Reveals a New Role of S4 Positively Charged Residues in PKD2L1 Channel Voltage-Sensing.Scientific reports · 2017Article
- Smooth Muscle Ion Channels and Regulation of Vascular Tone in Resistance Arteries and Arterioles.Comprehensive Physiology · 2017Review
- Ciliated neurons lining the central canal sense both fluid movement and pH through ASIC3.Nature communications · 2016Article
- Acid-induced off-response of PKD2L1 channel in Xenopus oocytes and its regulation by Ca(2.).Scientific reports · 2015Article
- PKD2L1/PKD1L3 channel complex with an alkali-activated mechanism and calcium-dependent inactivation.European biophysics journal : EBJ · 2015Article
- A proton current associated with sour taste: distribution and functional properties.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Gating modulation by heat of the polycystin transient receptor potential channel PKD2L1 (TRPP3).Pflugers Archiv : European journal of physiology · 2014Article
- Investigation of spinal cerebrospinal fluid-contacting neurons expressing PKD2L1: evidence for a conserved system from fish to primates.Frontiers in neuroanatomy · 2014Article
- The response of PKD1L3/PKD2L1 to acid stimuli is inhibited by capsaicin and its pungent analogs.The FEBS journal · 2012Article
- Molecular mechanism of the assembly of an acid-sensing receptor ion channel complex.Nature communications · 2012Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polycystic kidney disease 2-like 1(PKD2L1), previously called transient receptor potential polycystin 3 (TRPP3), forms constitutively active voltage-dependent nonselective cation channels in the plasma membrane. The mechanism of regulation of PKD2L1 channels, however, has been poorly understood. In the present study, we found a bell-shaped alkaline pH dependence of PKD2L1 channel activity at the single-channel and whole-cell levels in patch-clamp recordings in HEK293T cells overexpressing mouse PKD2L1: alkalization to pH 8.0-9.0 increased the PKD2L1 currents, but alkalization to pH 10.0 decreased them. Single-channel analysis revealed that alkalization changed the open probability of PKD2L1 channels, but not their single-channel conductance. In addition, the voltage dependence of PKD2L1 channels was negatively and positively shifted by treatment with solutions of pH 8.0-9.0 and pH 10.0, respectively. These results indicate that the voltage-dependent gating of PKD2L1 channels was modulated by alkalization through two different mechanisms. Interestingly, we observed rebound activation of the PKD2L1 channel on washout of the alkaline solution after PKD2L1 channel inhibition at pH 10.0, suggesting that alkalization to pH 10.0 decreased PKD2L1 currents by inactivating the channels. Consistently, the PKD2L1 tail currents were accelerated by alkalization. These results suggest that alkalization is a bimodal modulator of mouse PKD2L1 channels.
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