ArticleCell adhesion & migration
Innovative method for quantification of cell-cell adhesion in 96-well plates.
Article in Cell adhesion & migration. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 15 citations in OpenAlex.
- Article
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- Cloning, Expression and Inhibitory Effects on Lewis Lung Carcinoma Cells of rAj-Tspin from Sea Cucumber (Molecules (Basel, Switzerland) · 2021Article
- Natural Compounds as Target Biomolecules in Cellular Adhesion and Migration: From Biomolecular Stimulation to Label-Free Discovery and Bioactivity-Based Isolation.Biomedicines · 2021Review
- Epstein-Barr Virus Induces Expression of the LPAM-1 Integrin in B CellsJournal of virology · 2019Article
- Cytohesin 1 regulates homing and engraftment of human hematopoietic stem and progenitor cells.Blood · 2017Article
- Microenvironmental interactions between endothelial and lymphoma cells: a role for the canonical WNT pathway in Hodgkin lymphoma.Leukemia · 2017Article
- WNT5A: a motility-promoting factor in Hodgkin lymphoma.Oncogene · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell adhesion is an important part of many complex biological processes. It plays crucial roles in cancer, development, and maintenance of stem cell compartment. The measurement of adhesion under experimental conditions might provide important information for cell biology. There are several protocols to measure adhesion, usually based on washing or shaking to remove non-adherent cells. Here, we describe a quantification method based on gravitational force to measure adhesion in a 96-well format. Non-adherent cells are separated and only vital cells are quantified with a colorimetric assay. As example we provide the quantification of cell-cell interaction with blocking function antibodies for CD44, an N-cadherin antagonists and the stromal cell derived factor-1 alpha (SDF-1). This method facilitates fast and reliable measurement of cell adhesion in multiwell format for screening assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.