ArticleThe Journal of biological chemistry2011
Architecture and assembly of a divergent member of the ParM family of bacterial actin-like proteins.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Polymer dynamics of Alp7A reveals how two critical concentrations govern assembly of dynamically unstable actin-like proteins.Molecular biology of the cell · 2024Article
- On the critical concentration for net assembly of dynamically unstable polymers.bioRxiv : the preprint server for biology · 2024Article
- Article
- Plasmid Localization and Partition inEcoSal Plus · 2019Review
- Prokaryotic cytoskeletons: protein filaments organizing small cells.Nature reviews. Microbiology · 2018Review
- Bacterial Tubulins A and B Exhibit Polarized Growth, Mixed-Polarity Bundling, and Destabilization by GTP Hydrolysis.Journal of bacteriology · 2017Article
- In vitro assembly of the bacterial actin protein MamK from ' Candidatus Magnetobacterium casensis' in the phylum Nitrospirae.Protein & cell · 2016Article
- Effects of polymerization and nucleotide identity on the conformational dynamics of the bacterial actin homolog MreB.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Accessory factors promote AlfA-dependent plasmid segregation by regulating filament nucleation, disassembly, and bundling.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Bacterial actin-like proteins: purification and characterization of self-assembly properties.Methods in enzymology · 2014Article
- Bacterial actins and their diversity.Biochemistry · 2013Review
- Structure of the ParM filament at 8.5Å resolution.Journal of structural biology · 2013Article
- The bacterial actin MamK: in vitro assembly behavior and filament architecture.The Journal of biological chemistry · 2013Article
- The bacterial cytoskeleton: more than twisted filaments.Current opinion in cell biology · 2013Review
- Controlling the cortical actin motor.Protoplasma · 2012Review
- Article
- Alp7R regulates expression of the actin-like protein Alp7A in Bacillus subtilis.Journal of bacteriology · 2012Article
- MamK, a bacterial actin, forms dynamic filaments in vivo that are regulated by the acidic proteins MamJ and LimJ.Molecular microbiology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Eubacteria and archaea contain a variety of actin-like proteins (ALPs) that form filaments with surprisingly diverse architectures, assembly dynamics, and cellular functions. Although there is much data supporting differences between ALP families, there is little data regarding conservation of structure and function within these families. We asked whether the filament architecture and biochemical properties of the best-understood prokaryotic actin, ParM from plasmid R1, are conserved in a divergent member of the ParM family from plasmid pB171. Previous work demonstrated that R1 ParM assembles into filaments that are structurally distinct from actin and the other characterized ALPs. They also display three biophysical properties thought to be essential for DNA segregation: 1) rapid spontaneous nucleation, 2) symmetrical elongation, and 3) dynamic instability. We used microscopic and biophysical techniques to compare and contrast the architecture and assembly of these related proteins. Despite being only 41% identical, R1 and pB171 ParMs polymerize into nearly identical filaments with similar assembly dynamics. Conservation of the core assembly properties argues for their importance in ParM-mediated DNA segregation and suggests that divergent DNA-segregating ALPs with different assembly properties operate via different mechanisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.