ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2011
Sensitive immunofluorescent staining of cells via generation of fluorescent nanoscale polymer films in response to biorecognition.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Switchable DNA Photocatalysts for Radical Polymerization Controlled by Chemical Stimuli.Journal of the American Chemical Society · 2023Article
- Cell Death Persists in Rapid Extrusion of Lysis-Resistant Coated Cardiac Myoblasts.Bioprinting (Amsterdam, Netherlands) · 2020Article
- Coatings on mammalian cells: interfacing cells with their environment.Journal of biological engineering · 2019Review
- Comparison of eosin and fluorescein conjugates for the photoinitiation of cell-compatible polymer coatings.PloS one · 2018Article
- LY333531, a PKCβ inhibitor, attenuates glomerular endothelial cell apoptosis in the early stage of mouse diabetic nephropathy via down-regulating swiprosin-1.Acta pharmacologica Sinica · 2017Article
- The Role of Surface Receptor Density in Surface-Initiated Polymerizations for Cancer Cell Isolation.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Protective Polymer Coatings for High-Throughput, High-Purity Cellular Isolation.ACS applied materials & interfaces · 2015Article
- Characterization of molecular transport in ultrathin hydrogel coatings for cellular immunoprotection.Biomacromolecules · 2015Article
- Article
- Bioinformatics tools allow targeted selection of chromosome enumeration probes and aneuploidy detection.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2013Article
- Antigen-responsive, microfluidic valves for single use diagnostics.Lab on a chip · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Immunofluorescent staining is central to nearly all cell-based research, yet only a few fluorescent signal amplification approaches for cell staining exist, each with distinct limitations. Here, the authors present a novel, fluorescent polymerization-based amplification (FPBA) method that is shown to enable similar signal intensities as the highly sensitive, enzyme-based tyramide signal amplification (TSA) approach. Being non-enzymatic, FPBA is not expected to suffer from nonspecific staining of endogenous enzymes, as occurs with enzyme-based approaches. FPBA employs probes labeled with photopolymerization initiators, which lead to the controlled formation of fluorescent polymer films only at targeted biorecognition sites. Nuclear pore complex proteins (NPCs; in membranes), vimentin (in filaments), and von Willebrand factor (in granules) were all successfully immunostained by FPBA. Also, FPBA was demonstrated to be capable of multicolor immunostaining of multiple antigens. To assess relative sensitivity, decreasing concentrations of anti-NPC antibody were used, indicating that both FPBA and TSA stained NPC down to a 1:100,000 dilution. Nonspecific, cytoplasmic signal resulting from NPC staining was found to be reduced up to 5.5-fold in FPBA as compared to TSA, demonstrating better signal localization with FPBA. FPBA's unique approach affords a combination of preferred attributes, including high sensitivity and specificity not otherwise available with current techniques.
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Registered trials
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