ArticleJournal of microbiological methods2011
Current physical and SDS extraction methods do not efficiently remove exosporium proteins from Bacillus anthracis spores.
Article in Journal of microbiological methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Role of novel polysaccharide layers in assembly of the exosporium, the outermost protein layer of the Bacillus anthracis spore.Molecular microbiology · 2022Article
- Optimization of sporulation and purification methods for sporicidal efficacy assessment on Bacillus spores.Journal of industrial microbiology & biotechnology · 2022Article
- LysPBC2, a Novel Endolysin Harboring a Bacillus cereus Spore Binding Domain.Applied and environmental microbiology · 2019Article
- Observations on research with spores of Bacillales and Clostridiales species.Journal of applied microbiology · 2019Review
- Assembly of the outermost spore layer: pieces of the puzzle are coming together.Molecular microbiology · 2017Article
- The Exosporium Layer of Bacterial Spores: a Connection to the Environment and the Infected Host.Microbiology and molecular biology reviews : MMBR · 2015Review
- A genetic approach for the identification of exosporium assembly determinants of Bacillus anthracis.Journal of microbiological methods · 2013Article
- Assembly of the BclB glycoprotein into the exosporium and evidence for its role in the formation of the exosporium 'cap' structure in Bacillus anthracis.Molecular microbiology · 2012Article
- Surface layers of Clostridium difficile endospores.Journal of bacteriology · 2011Article
- Localization and assembly of the novel exosporium protein BetA of Bacillus anthracis.Journal of bacteriology · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Biochemical studies of the outermost spore layers of the Bacillus cereus family are hindered by difficulties in efficient dispersal of the external spore layers and difficulties in dissociating protein complexes that comprise the exosporium layer. Detergent and physical methods have been utilized to disrupt the exosporium layer. Herein we compare commonly used SDS extraction buffers used to extract spore proteins and demonstrate the incomplete extractability of the exosporium layer by these methods. Sonication and bead beating methods for exosporium layer removal were also examined. A combination of genetic and physical methods is the most effective for isolating proteins found in the spore exosporium.
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Registered trials
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