ArticleAnalytical chemistry2011
A simple and robust method for determining the number of basic sites in peptides and proteins using electrospray ionization mass spectrometry.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Direct ESI-MS of Ionic Liquids Using High-Pressure Electrospray From Large-Bore Emitters Made of Micropipette Tips.Mass spectrometry (Tokyo, Japan) · 2024Article
- Top-down mass spectrometry of intact phosphorylated β-casein: Correlation between the precursor charge state and internal fragments.Journal of mass spectrometry : JMS · 2019Article
- Flame Atmospheric Pressure Chemical Ionization Coupled with Negative Electrospray Ionization Mass Spectrometry for Ion Molecule Reactions.Journal of the American Society for Mass Spectrometry · 2017Article
- Effective protein separation by coupling hydrophobic interaction and reverse phase chromatography for top-down proteomics.Analytical chemistry · 2014Article
- Insights into the mechanism of protein electrospray ionization from salt adduction measurements.Journal of the American Society for Mass Spectrometry · 2014Article
- Cation-induced stabilization of protein complexes in the gas phase: mechanistic insights from hemoglobin dissociation studies.Journal of the American Society for Mass Spectrometry · 2014Article
- Dramatically stabilizing multiprotein complex structure in the absence of bulk water using tuned Hofmeister salts.Faraday discussions · 2013Article
- Solution additives that desalt protein ions in native mass spectrometry.Analytical chemistry · 2012Article
- Bound cations significantly stabilize the structure of multiprotein complexes in the gas phase.Angewandte Chemie (International ed. in English) · 2012Article
- Ion mobility-mass spectrometry for structural proteomics.Expert review of proteomics · 2012Review
- A new model for multiply charged adduct formation between peptides and anions in electrospray mass spectrometry.Journal of the American Society for Mass Spectrometry · 2011Article
- Effects of select anions from the Hofmeister series on the gas-phase conformations of protein ions measured with traveling-wave ion mobility spectrometry/mass spectrometry.Journal of the American Society for Mass Spectrometry · 2011Article
- Anion effects on sodium ion and acid molecule adduction to protein ions in electrospray ionization mass spectrometry.Journal of the American Society for Mass Spectrometry · 2011Article
- Bound anions differentially stabilize multiprotein complexes in the absence of bulk solvent.Journal of the American Chemical Society · 2011Article
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Authors and funding
3 authors.
Funding
Abstract
A solution additive has been discovered that can be used to measure the number of basic sites in a peptide or protein using electrospray ionization (ESI) mass spectrometry. Addition of millimolar amounts of perchloric acid (HClO(4)) to aqueous solutions that contain peptides or proteins results in the noncovalent adduction of HClO(4) molecules to the multiply charged ions formed by ESI. For 18 oligopeptides and proteins, ranging in molecular weight from 0.5 to 18.3 kDa, the sum of the number of protons plus maximum number of HClO(4) molecules adducted to the lower charge state ions is equal to the number of basic sites in the molecule. This method provides a rapid means of obtaining information about the composition of a peptide or protein and does not require high-resolution measurements or any instrumental or experimental modifications.
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