ArticleMolecular bioSystems2011
LIPS arrays for simultaneous detection of antibodies against partial and whole proteomes of HCV, HIV and EBV.
Article in Molecular bioSystems, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Ultrarapid Measurement of Diagnostic Antibodies by Magnetic Capture of Immune Complexes.Scientific reports · 2017Trial
- Profiling Norovirus Antibody Specificity and Function Using Luciferase Immunoprecipitation System (LIPS) Assays.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Are HIV-1-Specific Antibody Levels Potentially Useful Laboratory Markers to Estimate HIV Reservoir Size? A Review.Frontiers in immunology · 2021Review
- Lethal Hemorrhagic Disease and Clinical Illness Associated with Elephant Endotheliotropic Herpesvirus 1 Are Caused by Primary Infection: Implications for the Detection of Diagnostic Proteins.Journal of virology · 2020Article
- Inherited Chromosomally Integrated Human Herpesvirus 6 Demonstrates Tissue-Specific RNA ExpressionJournal of virology · 2019Article
- Emerging technologies for the detection of viral infections.Future virology · 2019Article
- Anti-Human Immunodeficiency Virus Antibodies in the Cerebrospinal Fluid: Evidence of Early Treatment Impact on Central Nervous System Reservoir?The Journal of infectious diseases · 2018Article
- Kinetics of Epstein-Barr Virus (EBV) Neutralizing and Virus-Specific Antibodies after Primary Infection with EBV.Clinical and vaccine immunology : CVI · 2016Article
- Luciferase immunoprecipitation systems for measuring antibodies in autoimmune and infectious diseases.Translational research : the journal of laboratory and clinical medicine · 2015Review
- Canine hepacivirus is not associated with chronic liver disease in dogs.Journal of viral hepatitis · 2014Article
- Viral surveillance and discovery.Current opinion in virology · 2013Review
- Article
- Serology-enabled discovery of genetically diverse hepaciviruses in a new host.Journal of virology · 2012Article
- Article
- No serological evidence for a role of HHV-6 infection in chronic fatigue syndrome.American journal of translational research · 2012Article
- The Cancer-Associated Virus Landscape in HIV Patients with Oral Hairy Leukoplakia, Kaposi's Sarcoma, and Non-Hodgkin Lymphoma.AIDS research and treatment · 2012Article
- Microfluidic LIPS for serum antibody detection: demonstration of a rapid test for HSV-2 infection.Biomedical microdevices · 2011Article
- Emerging biomedical applications of synthetic biology.Nature reviews. Genetics · 2011Review
- Searching for biomarkers: humoral response profiling with luciferase immunoprecipitation systems.Expert review of proteomics · 2011Review
- Serological studies confirm the novel astrovirus HMOAstV-C as a highly prevalent human infectious agent.PloS one · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
For many infectious agents, the detection of antibodies is critical for diagnosing, monitoring and understanding vaccine responses. To facilitate the highly quantitative and simultaneous analysis of antibodies against multiple proteins from infectious agents, we have developed Luciferase Immunoprecipitation Systems (LIPS) arrays. By configuring microtiter plates with multiple antigens and testing control and infected serum samples at one time in solution, LIPS arrays provided highly reproducible antibody titers to panels of antigens with a wide dynamic range of detection. While all serum samples showed similar positive and negative immunoreactivity with internal control antigens derived from Influenza and Renilla luciferase-alone protein, respectively, antibody titers to many HCV and HIV antigens were generally 10 to over 400-fold higher in the infected versus uninfected samples. Additional screening of 18 proteins from the EBV proteome with serum samples from healthy EBV-infected individuals showed statistically significant antibody titers to 50% of the proteins tested. Antibody titers for the different EBV antigens in the healthy EBV-infected individuals were markedly heterogeneous highlighting the complexity of host humoral responses. These results suggest that LIPS arrays offer a highly discriminating platform for simultaneously profiling a wide spectrum of antibodies associated with many infectious agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.