ArticleBiochimica et biophysica acta2011
Disrupting microtubule network immobilizes amoeboid chemotactic receptor in the plasma membrane.
Article in Biochimica et biophysica acta, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Field model for multistate lateral diffusion of various transmembrane proteins observed in living Dictyostelium cells.Journal of cell science · 2023Article
- Maximum likelihood-based estimation of diffusion coefficient is quick and reliable method for analyzing estradiol actions on surface receptor movements.Frontiers in neuroinformatics · 2023Article
- Different Heterotrimeric G Protein Dynamics for Wide-Range Chemotaxis in Eukaryotic Cells.Frontiers in cell and developmental biology · 2021Review
- The cAMP-induced G protein subunits dissociation monitored in live Dictyostelium cells by BRET reveals two activation rates, a positive effect of caffeine and potential role of microtubules.Cellular signalling · 2018Article
- Turnover and flow of the cell membrane for cell migration.Scientific reports · 2017Article
- Cargo binding promotes KDEL receptor clustering at the mammalian cell surface.Scientific reports · 2016Article
- Single Molecule Imaging Deciphers the Relation between Mobility and Signaling of a Prototypical G Protein-coupled Receptor in Living Cells.The Journal of biological chemistry · 2015Article
- TrackArt: the user friendly interface for single molecule tracking data analysis and simulation applied to complex diffusion in mica supported lipid bilayers.BMC research notes · 2014Article
- Independent localization of plasma membrane and chloroplast components during eyespot assembly.Eukaryotic cell · 2013Article
- The multiple faces of prostaglandin E2 G-protein coupled receptor signaling during the dendritic cell life cycle.International journal of molecular sciences · 2013Review
- SMAD versus non-SMAD signaling is determined by lateral mobility of bone morphogenetic protein (BMP) receptors.The Journal of biological chemistry · 2012Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Signaling cascades are initiated in the plasma membrane via activation of one molecule by another. The interaction depends on the mutual availability of the molecules to each other and this is determined by their localization and lateral diffusion in the cell membrane. The cytoskeleton plays a very important role in this process by enhancing or restricting the possibility of the signaling partners to meet in the plasma membrane. In this study we explored the mode of diffusion of the cAMP receptor, cAR1, in the plasma membrane of Dictyostelium discoideum cells and how this is regulated by the cytoskeleton. Single-particle tracking of fluorescently labeled cAR1 using Total Internal Reflection Microscopy showed that 70% of the cAR1 molecules were mobile. These receptors showed directed motion and we demonstrate that this is not because of tracking along the actin cytoskeleton. Instead, destabilization of the microtubules abolished cAR1 mobility in the plasma membrane and this was confirmed by Fluorescence Recovery after Photobleaching. As a result of microtubule stabilization, one of the first downstream signaling events, the jump of the PH domain of CRAC, was decreased. These results suggest a role for microtubules in cAR1 dynamics and in the ability of cAR1 molecules to interact with their signaling partners.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.