ArticleBiomaterials2011
High-throughput screening of microscale pitted substrate topographies for enhanced nonviral transfection efficiency in primary human fibroblasts.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 43 citations in OpenAlex.
- Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction.Journal of nanobiotechnology · 2025Article
- Cellular nanomechanics derived from pattern-dependent focal adhesion and cytoskeleton to balance gene transfection of malignant osteosarcoma.Journal of nanobiotechnology · 2022Article
- Designing electrospun fiber platforms for efficient delivery of genetic material and genome editing tools.Advanced drug delivery reviews · 2022Review
- High-Aspect-Ratio Nanostructured Surfaces as Biological Metamaterials.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Cellular and Subcellular Contact Guidance on Microfabricated Substrates.Frontiers in bioengineering and biotechnology · 2020Review
- Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Nanotopographical Modulation of Cell Function through Nuclear Deformation.ACS applied materials & interfaces · 2016Article
- Chitosan/siCkip-1 biofunctionalized titanium implant for improved osseointegration in the osteoporotic condition.Scientific reports · 2015Article
- 3-D Scaffold Platform for Optimized Non-viral Transfection of Multipotent Stem Cells.Journal of materials chemistry. B · 2014Article
- Surface-mediated nucleic acid delivery by lipoplexes prepared in microwell arrays.Small (Weinheim an der Bergstrasse, Germany) · 2013Article
- Physical non-viral gene delivery methods for tissue engineering.Annals of biomedical engineering · 2013Review
- Inorganic coatings for optimized non-viral transfection of stem cells.Scientific reports · 2013Article
- Combining topographical and genetic cues to promote neuronal fate specification in stem cells.Biomacromolecules · 2012Review
- Dynamic topographical control of mesenchymal stem cells by culture on responsive poly(ε-caprolactone) surfaces.Advanced materials (Deerfield Beach, Fla.) · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Optimization of nonviral gene delivery typically focuses on the design of particulate carriers that are endowed with desirable membrane targeting, internalization, and endosomal escape properties. Topographical control of cell transfectability, however, remains a largely unexplored parameter. Emerging literature has highlighted the influence of cell-topography interactions on modulation of many cell phenotypes, including protein expression and cytoskeletal behaviors implicated in endocytosis. Using high-throughput screening of primary human dermal fibroblasts cultured on a combinatorial library of microscale topographies, we have demonstrated an improvement in nonviral transfection efficiency for cells cultured on dense micropit patterns compared to smooth substrates, as verified with flow cytometry. A 25% increase in GFP(+) cells was observed independent of proliferation rate, accompanied by SEM and confocal microscopy characterization to help explain the phenomenon qualitatively. This finding encourages researchers to investigate substrate topography as a new design consideration for the optimization of nonviral transfection systems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.